Hyperbranched dendron and methods of synthesis and use thereof

• US 7,153,905 B2
• Filed: 03/22/2004
• Issued: 12/26/2006
• Est. Priority Date: 03/21/2003
• Status: Expired due to Fees

• Current Assignee: General Hospital Corporation, The General Hospital Corporation (Mass General Brigham, Inc.)
• Original Assignee: The General Hospital Corporation (Mass General Brigham, Inc.)
• Inventors: Reichardt, Wilfried, Banerjee, Pallab, Weissleder, Ralph, Bogdanov, Alexei
• Primary Examiner(s): Seidleck, James J.
• Assistant Examiner(s): Asinovsky, Olga
• Application Number:
  US10/806,025
• Publication Number:
  US 20050079149A1
• Time in Patent Office: 1,009 Days
• Field of Search: 525/92.B, 525/178, 525/179, 525/330.5, 525/54, 424/78.08, 424/497, 424/498
• US Class Current: 525/92.00B
• CPC Class Codes: C08F 271/00 Macromolecular compounds obtained by polymerising monomers on to polymers of nitrogen-containing monomers as defined in group C08F26/00
• CPC Class Codes: C08F 283/00 Macromolecular compounds obtained by polymerising monomers on to polymers provided for in subclass C08G
• CPC Class Codes: C08F 283/04 on to polycarbonamides, polyesteramides or polyimides C08F283/004 takes precedence
• CPC Class Codes: C08G 73/0206 Polyalkylene(poly)amines
• CPC Class Codes: C08G 83/003 Dendrimers
• CPC Class Codes: C12N 15/87 Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
• CPC Class Codes: Y10T 428/31848 Next to cellulosic

Abstract

Hyperbranched dendron (HD) polymers are synthesized using low molecular weight polyethyleneimine (BPEI-L) as a core and used for gene delivery. The obtained polymers display low toxicity and efficient gene delivery at low nitrogen-to-phosphate (N/P) ratios. Using successive attachment of ethyleneimine moieties to a PEI core, the polymer has a lower relative ratio of linear-to-branched structures than in the core PEI. The more extensive branching enables the polymer to condense plasmid DNA into nanostructure complexes with a size of less than or equal to about 100 nm. The complexes are stable and efficient in transfecting cells in the presence of serum. Bioluminescent imaging of in vivo gene expression using a luciferase reporter gene performed in live mice showed gene expression in the liver and in submandibular lymph nodes.

Claims

1. A polymer comprising:

a surface defined by the polymer;

a branched polyethyleneimine (B-PEI) core within the surface;

a plurality of primary amine groups at the surface; and

a plurality of secondary and tertiary amine groups positioned at the core;

wherein the polymer is a flexible hyperbranched dendron polymer.

2. The polymer of claim 1, wherein the polymer has a molecular weight of greater than or equal to about 10 kD.

3. The polymer of claim 2, wherein the polymer has a molecular weight of less than or equal to about 25 kD.

4. The polymer of claim 3, wherein the polymer has a secondary to tertiary amine ratio of less than or equal to about 1.5 to 1.

5. The polymer of claim 4, wherein the polymer has a secondary to tertiary amine ratio of less than or equal to about 1.3 to 1.

6. The polymer of claim 4, wherein the polymer has a secondary to tertiary amine ration of greater than about 1.0 to 1.

7. The polymer of claim 4, wherein the polymer has a secondary to tertiary amine ration of greater than or equal to about 1.2 to 1.

8. The polymer of claim 1, wherein the polymer is made by a process comprising iterative attachment of ethyleneimine moieties to a branched polyethyleneimine core.

9. The polymer of claim 8, wherein the process increases the amount of secondary and tertiary amines in the polymer while maintaining a plurality of primary amines on the surface of the polymer.

10. The polymer of claim 8, wherein the process comprising iterative attachment of ethyleneimine moieties to a branched polyethyleneimine core comprises:

(a) reacting polyethyleneimine with chloroethyl amine;

(b) reacting the modified polyethyleneimine of step (a) with chloroethyl amine; and

(c) reacting the modified polyethyleneimine of step (b) with chloroethyl amine.

28. The polymer of claim 1, wherein the polymer is polydisperse.

11. A hyperbranched dendron polymer having a randomly branched structure, a molecular weight of about 10 to 25 kD, and a ratio of secondary to tertiary amine groups of less than or equal to about 1.5 to 1.

12. The polymer of claim 11, wherein the ratio of secondary to tertiary amine groups is less than or equal to about 1.3 to 1.

13. The polymer of claim 12, wherein the ratio of secondary to tertiary amine groups is greater than or equal to about 1.2 to 1.

14. The polymer of claim 12, wherein the molecular weight is greater than or equal to about 12 kD.

15. The polymer of claim 12, wherein the molecular weight is less than or equal to about 15 kD.

16. The polymer of claim 11, wherein the polymer comprises:

a surface defined by the polymer;

a polyethyleneimine core within the surface;

a plurality of primary amine groups at the surface; and

a plurality of secondary and tertiary amine groups positioned at the core.

17. The polymer of claim 11, wherein the polymer is made by a process comprising iterative attachment of ethyleneimine moieties to a branched polyethyleneimine core.

18. The polymer of claim 17, wherein the process increases the amount of secondary and tertiary amines in the polymer while maintaining a plurality of primary amines on a surface of the polymer.

19. The polymer of claim 18, wherein the process comprising iterative attachment of ethyleneimine moieties to a branched polyethyleneimine core comprises:

(a) reacting polyethyleneimine with chloroethyl amine;

(b) reacting the modified polyethyleneimine of step (a) with chloroethyl amine; and

(c) reacting the modified polyethyleneimine of step (b) with chloroethyl amine.

29. The polymer of claim 11, wherein the polymer is a flexible hyperbranched dendron polymer.

30. The polymer of claim 11, wherein the polymer is polydisperse.

20. A hyperbranched dendron polymer made by a process comprising iterative attachment of ethyleneimine moieties to a branched polyethyleneimine core, wherein the process increases the amount of secondary and tertiary amines in the polymer while maintaining a plurality of primary amines on a surface of the polymer.

21. The polymer of claim 20, wherein the process comprising iterative attachment of ethyleneimine moieties to a branched polyethyleneimine core comprises:

(a) reacting polyethyleneimine with chloroethyl amine;

(b) reacting the modified polyethyleneimine of step (a) with chloroethyl amine; and

(c) reacting the modified polyethyleneimine of step (b) with chloroethyl amine.

22. The polymer of claim 20, wherein the polymer has a molecular weight of greater than or equal to about 10 kD.

23. The polymer of claim 22, wherein the polymer has a molecular weight of less than or equal to about 25 kD.

24. The polymer of claim 23, wherein the polymer has a secondary to tertiary amine ratio of less than or equal to about 1.5 to 1.

25. The polymer of claim 24, wherein the polymer has a secondary to tertiary amine ratio of less than or equal to about 1.3 to 1.

26. The polymer of claim 24, wherein the polymer has a secondary to tertiary amine ration of greater than about 1.0 to 1.

27. The polymer of claim 24, wherein the polymer has a secondary to tertiary amine ration of greater than or equal to about 1.2 to 1.

31. The polymer of claim 20, wherein the polymer is a flexible hyperbranched dendron polymer.

32. The polymer of claim 20, wherein the polymer is polydisperse.