Tag nucleic acids and probe arrays

• US 7,157,564 B1
• Filed: 04/04/2001
• Issued: 01/02/2007
• Est. Priority Date: 04/06/2000
• Status: Active Grant

• Current Assignee: Affymetrix, Inc. (Thermo Fisher Scientific Incorporated)
• Original Assignee: Affymetrix, Inc. (Thermo Fisher Scientific Incorporated)
• Inventors: Morris, MacDonald, Lockhart, David, Ryder, Thomas B., Mittmann, Michael
• Primary Examiner(s): FREDMAN, JEFFREY NORMAN
• Application Number:
  US09/827,383
• Time in Patent Office: 2,099 Days
• Field of Search: 536/23.1, 536/24.3, 536/24.31, 536/24.33, 435/6, 435/810, 435/91.2
• US Class Current: 536/23.1
• CPC Class Codes: C12Q 1/6837 using probe arrays or probe chips C12Q1/6874 takes precedence
• CPC Class Codes: C12Q 2525/161 incorporating target specific and non-target specific sites
• CPC Class Codes: Y10T 436/13 Tracers or tags

Abstract

The invention provides a unique set of nucleic acid sequences which is appropriate for use for a wide variety of applications requiring nucleic acid tags. As such, the sequence tags of the presently claimed invention may be used, for example, to label biological and nonbiological materials, in genotyping applications and in a variety of other analyses.

Claims

1. A set of nucleic acid tags probes comprising at least 1000 nucleic acid sequences chosen from the group consisting of:

SEQ ID NOS;

1–

2000.

3. The set of nucleic acid tag-probes of claim 1 attached to a solid support.

2. A set of nucleic acid tags comprising at least 1000 nucleic acid sequences chosen from the group consisting of:

the complements of SEQ ID NOS;

1–

2000.

4. The set of nucleic acid tags of claim 2 attached to a solid support.

5. A set of nucleic acids comprising:

a first set of nucleic acids comprising each of the sequences listed in SEQ ID NO. 1–

2000,

a second set of nucleic acids comprising the complement of each of the first set of nucleic acids,

a third set of nucleic acids comprising the first set of nucleic acids wherein position 10 in each of SEQ ID NO;

1–

2000 is changed to a mismatch, and

a fourth set of nucleic acids comprising the complement of each of the third set of nucleic acids.

6. The set of nucleic acids of claim 5 attached to a solid support.

7. The set of nucleic acids of claim 5 further comprising a first set of nucleic acids comprising each of the sequences listed in SEQ ID Nos. 2001–

2050.

8. The set of nucleic acids of claim 7 attached to a solid support.