UV excitable energy transfer reagents
First Claim
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1. A fluorescently labeled reagent comprising:
- a reagent selected train the group consisting of a nucleoside, a nucleotide, an oligonucleotide and an oligonucleotide analog; and
an energy transfer fluorescent label attached thereto, the energy transfer fluorescent label comprising;
a donor dye having an absorption maximum at a wavelength between 250 and 400 nm; and
an acceptor dye capable of absorbing excitation energy emitted by the donor dye and emitting light in response, the acceptor dye having an emission maximum of greater than about 500 nm,whereby excitation of the donor dye with light at said absorption maximum causes said acceptor dye to emit light at said emission maximum.
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Abstract
Novel energy transfer dyes which can be used with shorter wavelength light sources are provided. These dyes include a donor dye with an absorption maxima at a wavelength between about 250 to 450 nm and an acceptor dye which is capable of absorbing energy emitted from the donor dye. One of the energy transfer dyes has a donor dye which is a member of a class of dyes having a coumarin or pyrene ring structure and an acceptor dye which is capable of absorbing energy emitted from the donor dye, wherein the donor dye has an absorption maxima between about 250 and 450 nm and the acceptor dye has an emission maxima at a wavelength greater than about 500 nm.
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Citations
28 Claims
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1. A fluorescently labeled reagent comprising:
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a reagent selected train the group consisting of a nucleoside, a nucleotide, an oligonucleotide and an oligonucleotide analog; and an energy transfer fluorescent label attached thereto, the energy transfer fluorescent label comprising; a donor dye having an absorption maximum at a wavelength between 250 and 400 nm; and an acceptor dye capable of absorbing excitation energy emitted by the donor dye and emitting light in response, the acceptor dye having an emission maximum of greater than about 500 nm, whereby excitation of the donor dye with light at said absorption maximum causes said acceptor dye to emit light at said emission maximum. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28)
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18. A fluorescently labeled reagent comprising:
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a reagent selected from the group consisting of a nucleoside, a nucleoside monophosphate, a nucleoside diphosphate, a nucleoside triphosphate, an oligonucleotide and an oligonucleotide analog; and an energy transfer fluorescent dye attached to the reagent, the energy transfer fluorescent dye being selected from the group consisting of DYE102, DYE104, DYE106, DYE108, DYE118, and DYE120.
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Specification