Compositions for the electronic detection of analytes utilizing monolayers

• US 7,160,678 B1
• Filed: 11/30/1999
• Issued: 01/09/2007
• Est. Priority Date: 11/05/1996
• Status: Expired due to Fees

• Current Assignee: Roche Molecular Systems (Roche Holding AG)
• Original Assignee: Clinical Micro Sensors Inc. (Roche Holding AG)
• Inventors: Kayyem, Jon Faiz, O'Connor, Stephen D.
• Primary Examiner(s): Whisenant; Ethan
• Application Number:
  US09/452,277
• Time in Patent Office: 2,597 Days
• Field of Search: 435/6, 435/91.1, 536/23.1
• US Class Current: 435/6.11
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: B82Y 40/00 Manufacture or treatment of nanostructures
• CPC Class Codes: C12Q 1/6825 Nucleic acid detection involving sensors
• CPC Class Codes: C12Q 2563/113 the label being electroactive, e.g. redox labels
• CPC Class Codes: C12Q 2565/519 characterised by the capture moiety being a single stranded oligonucleotide

Abstract

The present invention relates to the use of self-assembled monolayers with mixtures of conductive oligomers and insulators to detect target analytes.

Claims

1. A composition comprising:

a) an electrode comprising;

i) a monolayer comprising insulators; and

ii) a capture binding ligand wherein said capture binding ligand is covalently attached to said electrode via an attachment linker; and

,

b) a target analyte binding ligand comprising at least one non-electrochemiluminescent electron transfer moiety (ETM) covalently attached to said target analyte binding ligand.

2. A composition according to claim 1 wherein said attachment linker is selected from the group consisting of conductive oligomers and insulators.

3. A composition according to claim 1 wherein said capture binding ligand is a nucleic acid.

4. A composition according to claim 1 wherein said capture binding ligand is a protein.

5. A composition according to claim 1 wherein said ETM is a metallocene.

6. A composition according to claim 5 wherein said metallocene is ferrocene.

7. A composition according to claim 1 wherein said insulators are alkyl chains.

15. A composition according to claim 1 wherein said monolayer further comprises conductive oligomers.

17. A composition according to claim 2 wherein said attachment linker is a conductive oligomer.

18. A composition according to claim 2 wherein said attachment linker is an insulator.

8. A composition comprising:

a) an electrode comprising;

i) a monolayer comprising insulators; and

ii) a capture binding ligand wherein said capture binding ligand is covalently attached to said electrode via an attachment linker; and

,

b) a target analyte binding ligand comprising;

i) nucleic acid;

ii) at least one non-electrochemiluminescent electron transfer moiety (ETM) covalently attached to said target analyte binding ligand.

9. A composition according to claim 8 wherein said attachment linker is selected from the group consisting of conductive oligomers and insulators.

10. A composition according to claim 8 wherein said capture binding ligand is a nucleic acid.

11. A composition according to claim 8 wherein said capture binding ligand is a protein.

12. A composition according to claim 8 wherein said ETM is a metallocene.

13. A composition according to claim 12 wherein said metallocene is ferrocene.

14. A composition according to claim 8 wherein said insulators are alkyl chains.

16. A composition according to claim 8 wherein said monolayer further comprises conductive oligomers.

19. A composition according to claim 9 wherein said attachment linker is a conductive oligomer.

20. A composition according to claim 9 wherein said attachment liker is an insulator.