Compositions comprising conditioned cell culture media and uses thereof
First Claim
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1. A composition comprising:
- conditioned cell culture media, or an extract thereof, comprising at least one culture-derived growth factor, wherein the at least one growth factor comprises at least one of vascular endothelial growth factor (VEGF), transforming growth factor beta (TGFβ
), hepatocyte growth factor (HGF), keratinocyte growth factor (KGF), interleukin-3 (IL-3), IL-6, and IL-8;
at least one culture-derived antioxidant, wherein the at least one antioxidant comprises at least one of glutathione, glutathione peroxidase, glutathione reductase, glutathione disulfide, catalase, superoxide dismutase, alpha-tocopherol, gamma-tocopherol, ubiquinol-9, ubiquinone 9, ascorbic acid, cysteine, and cystine; and
at least one culture-derived soluble collagen; and
an appropriate carrier, and wherein the cell culture media is conditioned by human fibroblasts in three-dimensional culture.
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Abstract
The invention relates to compositions comprising cell culture medium conditioned by cells grown in three-dimensional culture. The cells used to condition the medium may be genetically modified to alter the concentration of growth factors and antioxidants in the medium. The conditioned cell medium (conditioned medium) may be used for at least one of cosmetic applications, cosmeceutical applications, and pharmaceutical applications, among other things. The invention also relates to proteins comprising a heterologous sequence that enhances cell penetration. The invention also relates to cells comprising DNA encoding such proteins. Methods for preparing the inventive compounds are also provided.
33 Citations
48 Claims
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1. A composition comprising:
- conditioned cell culture media, or an extract thereof, comprising at least one culture-derived growth factor, wherein the at least one growth factor comprises at least one of vascular endothelial growth factor (VEGF), transforming growth factor beta (TGFβ
), hepatocyte growth factor (HGF), keratinocyte growth factor (KGF), interleukin-3 (IL-3), IL-6, and IL-8;
at least one culture-derived antioxidant, wherein the at least one antioxidant comprises at least one of glutathione, glutathione peroxidase, glutathione reductase, glutathione disulfide, catalase, superoxide dismutase, alpha-tocopherol, gamma-tocopherol, ubiquinol-9, ubiquinone 9, ascorbic acid, cysteine, and cystine; and
at least one culture-derived soluble collagen; and
an appropriate carrier, and wherein the cell culture media is conditioned by human fibroblasts in three-dimensional culture. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
- conditioned cell culture media, or an extract thereof, comprising at least one culture-derived growth factor, wherein the at least one growth factor comprises at least one of vascular endothelial growth factor (VEGF), transforming growth factor beta (TGFβ
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21. A cosmeceutical composition comprising:
- cell culture media or an extract thereof, conditioned by incubation with a three-dimensional cell culture, wherein the conditioned media or extract comprises at least one culture-derived growth factor, the at least one growth factor comprising at least one of;
vascular endothelial growth factor (VEGF), transforming growth factor beta (TGFIβ
), hepatocyte growth factor (HGF), keratinocyte growth factor (KGF), interleukin-3 (IL-3), IL-6, and IL-8; and
at least one culture-derived antioxidant, the at least one antioxidant comprising at least one of;
glutathione, glutathione peroxidase, glutathione reductase, glutathione disulfide, catalase, superoxide dismutase, alpha-tocopherol, gamma-tocopherol, ubiquinol-9, ubiquinone 9, ascorbic acid, cysteine, and cystine; and
a cosmeceutically-acceptable carrier, and wherein the cell culture media is conditioned by human fibroblasts in three-dimensional culture. - View Dependent Claims (22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37)
- cell culture media or an extract thereof, conditioned by incubation with a three-dimensional cell culture, wherein the conditioned media or extract comprises at least one culture-derived growth factor, the at least one growth factor comprising at least one of;
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38. A method for preparing a cosmeceutical composition comprising:
- combining a pre-conditioned medium with a thee-dimensional cell culture under appropriate conditions to generate a conditioned medium comprising at least one culture-derived growth factor, the at least one growth factor comprising at least one of;
vascular endothelial growth factor (VEGF), transforming growth factor beta (TGFβ
), hepatocyte growth factor (HGF), keratinocyte growth factor (KGF), interleukin-3 (IL-3), IL-6, and IL-8; and
at least one culture-derived antioxidant, the at least one antioxidant comprising at least one of;
glutathione, glutathione peroxidase, glutathione reductase, glutathione disulfide, catalase, superoxide dismutase, alpha-tocopherol, gamma-tocopherol, ubiquinol-9, ubiquinone 9, ascorbic acid, cysteine, and cystine; and
combining the conditioned medium with a cosmeceutically-acceptable carrier to form a cosmeceutical composition, wherein the cell culture media is conditioned by human fibroblasts in three-dimensional culture, and wherein the at least one growth factor comprises at least one genetically-engineered transport-enhanced growth factor and/or and the at least one antioxidant comprises at least one genetically-engineered transport-enhanced antioxidant. - View Dependent Claims (41, 42, 43, 44, 45, 46, 47, 48)
- combining a pre-conditioned medium with a thee-dimensional cell culture under appropriate conditions to generate a conditioned medium comprising at least one culture-derived growth factor, the at least one growth factor comprising at least one of;
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39. A method for preparing a composition comprising:
- combining a pre-conditioned medium with a three-dimensional cell culture under appropriate conditions to generate a conditioned medium comprising at least one culture-derived growth factor, the at least one growth factor comprising at least one of;
vascular endothelial growth factor (VEGF), transforming growth factor beta (TGFβ
), hepatocyte growth factor (HGF), keratinocyte growth factor (KGF), interleukin-3 (IL-3), IL-6, and IL-8; and
at least one culture-derived antioxidant, the at least one antioxidant comprising at least one of;
glutathione, glutathione peroxidase, glutathione reductase, glutathione disulfide, catalase, superoxide dismutase, alpha-tocopherol, gamma-tocopherol, ubiquinol-9, ubiquinone 9, ascorbic acid, cysteine, and cystine; and
combining the conditioned medium with an acceptable carrier to form a composition, wherein the cell culture media is conditioned by human fibroblasts in three-dimensional culture, and wherein the at least one growth factor comprises at least one genetically-engineered transport-enhanced growth factor and/or and the at least one antioxidant comprises at least one genetically-engineered transport-enhanced antioxidant. - View Dependent Claims (40)
- combining a pre-conditioned medium with a three-dimensional cell culture under appropriate conditions to generate a conditioned medium comprising at least one culture-derived growth factor, the at least one growth factor comprising at least one of;
Specification