Retentate chromatography and protein chip arrays with applications in biology
First Claim
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1. A kit comprising:
- (1) at least one laser desorption/ionization mass spectrometry probe substrate comprising a plurality of different adsorbents, each adsorbent attached to the at least one substrate at different addressable locations;
(2) at least one container comprising at least one eluant; and
(3) instructions for detecting a plurality of polypeptides that are diagnostic markers by contacting the adsorbents with a sample comprising the polypeptides, capturing the polypeptides on the different adsorbents, washing the adsorbents with the at least one eluant, and detecting the captured polypeptides by laser desorption/ionization mass spectrometry.
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Abstract
This invention provides methods of retentate chromatography for resolving analytes in a sample. The methods involve adsorbing the analytes to a substrate under a plurality of different selectivity conditions, and detecting the analytes retained on the substrate by desorption spectrometry. The methods are useful in biology and medicine, including clinical diagnostics and drug discovery.
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10 Claims
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1. A kit comprising:
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(1) at least one laser desorption/ionization mass spectrometry probe substrate comprising a plurality of different adsorbents, each adsorbent attached to the at least one substrate at different addressable locations; (2) at least one container comprising at least one eluant; and (3) instructions for detecting a plurality of polypeptides that are diagnostic markers by contacting the adsorbents with a sample comprising the polypeptides, capturing the polypeptides on the different adsorbents, washing the adsorbents with the at least one eluant, and detecting the captured polypeptides by laser desorption/ionization mass spectrometry. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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Specification