Fluorescence energy transfer probes with stabilized conformations

• US 7,160,996 B1
• Filed: 06/08/2000
• Issued: 01/09/2007
• Est. Priority Date: 06/09/1999
• Status: Expired due to Fees

• Current Assignee: HSB Corporate Trust Company (UK) Limited
• Original Assignee: Bioresearch Technologies Incorporated
• Inventors: Cook, Ronald M.
• Primary Examiner(s): EPPS -SMITH, JANET L
• Application Number:
  US09/591,185
• Time in Patent Office: 2,406 Days
• Field of Search: 435/6, 435/5, 435/91.1, 435/93.1, 435/91.2, 536/24.3, 536/26.6, 536/23.1, 536/24.33, 536/22.1, 536/25.3, 536/25.32
• US Class Current: 536/24.3
• CPC Class Codes: C07H 21/04 with deoxyribosyl as saccharide radical
• CPC Class Codes: C12Q 1/6876 Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

Abstract

The present invention provides a class of Conformationally Assisted Probes comprising (a) a nucleic acid moiety; (b) an energy donor moiety; (c) an energy acceptor moiety; and (d) one or more stabilizing moieties.

Claims

1. A probe nucleic acid having the formula:

2. The probe nucleic acid according to claim 1, wherein R²—

CHOL and R³—

CHOL are independently selected and have structures according to the formula;

3. The probe nucleic acid according to claim 2, wherein said PEG has from about 2 to about 20 ethylene glycol subunits.

4. The probe nucleic acid according to claim 2 in which R¹¹ is substituted or unsubstituted alkyl.

5. The probe nucleic acid according to claim 3, wherein R¹¹ is C₁–

C₆ substituted or unsubstituted alkyl.

6. The probe nucleic acid according to claim 2, wherein Y³—

CHOL has the structure;

7. The probe nucleic acid according to claim 1, 2 wherein Nu¹ and Nu² are nucleotides having an exocyclic amine group to which —

R¹—

D and —

R⁴Q are attached, respectively.

8. A probe nucleic acid having the formula:

9. The probe nucleic acid according to claim 8, wherein Y¹ and Y² are members independently selected from substituted or unsubstituted heteroalkyl.

10. The probe nucleic acid according to claim 9, wherein Y¹ and Y² are polyethylene glycol.

11. The probe nucleic acid according to claim 10, wherein said polyethylene glycol has from about 2 to about 20 ethylene glycol subunits.

12. The probe nucleic acid according to claim 8, wherein Y¹—

CHOL and Y²—

CHOL have the structure;