Reagents and methods for detecting analytes, and devices comprising reagents for detecting analytes
First Claim
Patent Images
1. A reagent for detecting an analyte, comprising:
- an enzyme selected from the group consisting of a flavoprotein, a quinoprotein, and a combination thereof; and
a mediator selected from the group
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Abstract
Reagents for detecting an analyte are described. A reagent comprises (a) an enzyme selected from the group consisting of a flavoprotein, a quinoprotein, and a combination thereof; and (b) a mediator selected from the group consisting of a phenothiazine, a phenoxazine, and a combination thereof. In addition, reagents having good stability to radiation sterilization are described. Electrochemical sensors and sampling devices comprising such reagents, methods of producing a sterilized device including such reagents, and methods for detecting an analyte which utilize such reagents are described as well.
202 Citations
40 Claims
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1. A reagent for detecting an analyte, comprising:
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an enzyme selected from the group consisting of a flavoprotein, a quinoprotein, and a combination thereof; and a mediator selected from the group - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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12. A reagent for detecting glucose, comprising:
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PQQ-glucose dehydrogenase in an activity of about 20 Units/μ
L;a buffer having a concentration between about 0.1 mM and about 100 mM, and a pH between about 4.5 and about 9.5; and a mediator having a structure - View Dependent Claims (13, 14, 15)
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16. A reagent for detecting an analyte, comprising:
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an enzyme selected from the group consisting of PQQ-glucose dehydrogenase, FAD-glucose oxidase, and a combination thereof; and a mediator selected from the group consisting of - View Dependent Claims (17, 18)
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19. An electrochemical sensor comprising:
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a working electrode having a surface; and a second electrode coupled to the working electrode, wherein the surface of the working electrode is coated with a solution of a reagent comprising an enzyme selected from the group consisting of a flavoprotein, a quinoprotein, and a combination thereof; and
a mediator selected fromand combinations thereof, - View Dependent Claims (20, 21, 22, 23)
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24. An electrochemical sensor comprising:
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a working electrode having a surface; and a reference electrode coupled to the working electrode, wherein the surface of the working electrode is coated with a solution of a reagent comprising PQQ-glucose dehydrogenase in an activity of about 1 Units/μ
L to about 100 Units/μ
L;a buffer having a concentration of about 100 mM and a pH of about 7.4; and a mediator having a structure - View Dependent Claims (25, 26, 27)
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28. A device for measuring an analyte, comprising:
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a lancet; and a sampling chamber connected to the lancet, comprising; a reagent comprising; an enzyme selected from the group consisting of PQQ-glucose dehydrogenase, FAD-glucose oxidase, and a combination thereof; and a mediator selected from - View Dependent Claims (29, 30, 31)
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32. A method for detecting an analyte which undergoes a chemical reaction, the method comprising:
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providing an electrode surface; catalyzing the chemical reaction with an enzyme selected from the group consisting of a flavoprotein, a quinoprotein, and a combination thereof; generating a redox equivalent by the chemical reaction; and transferring the redox equivalent to the electrode surface using a mediator selected from - View Dependent Claims (33, 34, 35, 36, 37, 38, 39, 40)
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Specification
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Current AssigneeAscensia Diabetes Care Holdings AG (PHC Holdings Corporation)
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Original AssigneeBayer Corporation (Bayer AG)
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InventorsWarchal-Windham, Mary Ellen, Vreeke, Mark S., Blaschke, Christina, Mikel, Barbara J., Cooper, Howard A.
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Primary Examiner(s)Olsen; Kaj K.
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Application NumberUS10/231,539Publication NumberTime in Patent Office1,596 DaysField of Search204/403, 204/409, 204/412, 204/403.14, 205/775, 205/777.5, 435/14, 435/25, 435/26, 435/810, 435/817, 436/806, 436/808, 436/149, 436/151US Class Current205/777.5CPC Class CodesC12Q 1/32 involving dehydrogenaseC12Q 1/54 involving glucose or galactose
