Red blood cell separation method

• US 7,166,231 B2
• Filed: 01/09/2003
• Issued: 01/23/2007
• Est. Priority Date: 09/03/1999
• Status: Expired due to Term

• Current Assignee: Fenwal Incorporated (Fresenius SE & Co. KGaA)
• Original Assignee: Baxter International Inc.
• Inventors: Westberg, Tom, Joie, Michel L. F., Smith, Kelly B
• Primary Examiner(s): Menon; Krishnan S.
• Application Number:
  US10/339,473
• Publication Number:
  US 20030203801A1
• Time in Patent Office: 1,475 Days
• Field of Search: 210/787, 210/789, 494/37, 494/43, 494/45
• US Class Current: 210/787
• CPC Class Codes: B01D 21/0003 Making of sedimentation devices, structural details thereof, e.g. prefabricated parts
• CPC Class Codes: B01D 21/26 Separation of sediment aided by centrifugal force or centripetal forcecentrifuges B04B; cyclones B04C
• CPC Class Codes: B01D 2221/10 Separation devices for use in medical, pharmaceutical or laboratory applications, e.g. separating amalgam from dental treatment residues
• CPC Class Codes: B04B 2005/045 having annular separation channels
• CPC Class Codes: B04B 2005/0492 with fluid conveying umbilicus between stationary and rotary centrifuge parts
• CPC Class Codes: B04B 5/0442 with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation

Abstract

A method for centrifugally separating whole blood into red blood cells and a plasma constituent rotates about a rotational axis a separation zone having radially spaced apart walls with a high-G side and a low-G side located closer to the rotational axis than the high-G side. Whole blood enters the rotating separation zone in an entry region to begin separation. Separation is halted by a terminal wall that is circumferentially spaced from the entry region. The whole blood separates into red blood cells toward the high-G side and plasma constituent toward the low-G side. The method directs red blood cells separated in the separation zone in a circumferential flow direction toward the terminal wall. The method directs separated red blood cells from the rotating separation zone through a second path that extends, at least in part, radially beyond the high-G wall.

Claims

1. A method for centrifugally separating whole blood into red blood cells and a plasma constituent comprising the steps of

rotating a separation zone about a rotational axis, the rotating separation zone having radially spaced apart walls with a high-G side and a low-G side located closer to the rotational axis than the high-G side, the spaced apart walls defining a blood flow path that extends circumferentially about the rotation axis, the rotating separation zone including an entry region where whole blood enters the rotating separation zone to begin separation and a terminal wall that is circumferentially spaced from the entry region, where flow is halted,

directing whole blood into the rotating separation zone through a first path adjacent the entry region, to begin separation of the whole blood into red blood cells toward the high-G side and plasma constituent toward the low-G side,

directing red blood cells separated in the separation zone in a circumferential flow direction toward the terminal wall, and

directing separated red blood cells from the rotating separation zone through a second path that communicates with the separation zone adjacent the terminal wall such that substantial plasma constituent can not flow circumferentially past or into the second path, the second path including a portion that is recessed into the high-G wall.

2. A method according to claim 1 further including the step of directing separated plasma constituent from the rotating separation zone through a third path.

3. A method according to claim 1 further including the step of directing separated plasma constituent from the rotating separation zone through a third path that is spaced closer to the terminal wall than the third path is spaced from the first path.

4. A method according to claim 1 further including the step of directing separated plasma constituent from the rotating separation zone through a third path that is between the first path and the second path.

5. A method for centrifugally separating whole blood into red blood cells and a plasma constituent comprising the steps of

rotating a separation zone about a rotational axis, the rotating separation zone having radially spaced apart walls with a high-G side and a low-G side located closer to the rotational axis than the high-G side, a blood flow path that extends circumferentially about the rotation axis, the rotating separation zone including an entry region where whole blood enters the rotating separation zone to begin separation and a terminal wall that is circumferentially spaced from the entry region, where flow is halted,

directing whole blood into the rotating separation zone through a first path adjacent the entry region, to begin separation of the whole blood into red blood cells toward the high-G side and plasma constituent toward the low-G side,

directing red blood cells separated in the separation zone in a circumferential flow direction toward the terminal wall, and

directing separated red blood cells from the rotating separation zone through a second path that extends, at least in part, radially beyond the high-G wall of an upstream separation zone, said part of the second path including a portion which defines a constricted channel that allows red blood cells to pass through the second path but substantially prevents the flow of plasma through the second path.

6. A method according to claim 5 further including the step of directing separated plasma constituent from the rotating separation zone through a third path.

7. A method according to claim 5 further including the step of directing separated plasma constituent from the rotating separation zone through a third path that is between the first path and the second path.

8. A method for centrifugally separating whole blood into red blood cells and a plasma constituent comprising the steps of:

rotating a separation zone about a rotational axis, the rotating separation zone having radially spaced apart walls with a high-G side and a low-G side located closer to the rotational axis than the high-G side, the spaced apart walls defining a blood flow path that extends circumferentially about the rotation axis, the rotating separation zone including an entry region where whole blood enters the rotating separation zone to begin separation and a terminal wall that is circumferentially spaced from the entry region, where flow is halted,

directing whole blood into the rotating separation zone through a first path adjacent the entry region, to begin separation of the whole blood into red blood cells toward the high-G side and plasma constituent toward the low-G side,

directing red blood cells separated in the separation zone in a circumferential flow direction toward the terminal wall, and

directing separated red blood cells from the rotating separation zone, in a circumferential flow direction, through a second path that extends, at least in part, radially beyond the high-G wall of an upstream separation zone such that the red blood cells pass through and beyond at least said part of second path and the second path substantially prevents the flow of plasma therethrough.

9. A method according to claim 8 further including the step of directing separated plasma constituent from the rotating separation zone through a third path.

10. A method according to claim 8 further including the step of directing separated plasma constituent from the rotating separation zone through a third path that is between the first path and the second path.

11. A method for centrifugally separating a biological fluid into at least one higher density component and at least one lower density component, which includes plasma, the method comprising the steps of:

rotating a separation zone about a rotational axis, the rotating separation zone having radially spaced apart walls with a high-G side and a low-G side located closer to the rotational axis than the high-G side, the spaced apart walls defining a blood flow path that extends circumferentially about the rotational axis, the rotating separation zone including an entry region where the biological fluid enters the rotating separation zone to begin separation and a terminal wall that is circumferentially spaced from the entry region,

flowing the biological fluid into this rotating separation zone through a first path adjacent the entry region, to begin separation of the biological fluid into said at least one higher density component toward the high-G side and said at least one lower density component toward the low-G side,

flowing said at least one higher density component separated in the separation zone in a circumferential flow direction toward the terminal wall,

removing said at least one lower density component from the rotating separation zone through a second path that terminates at the low-G wall, and

removing said at least one higher density component from the rotating separation zone through a third path located closer to the terminal wall than the second path, the third path including a portion that is recessed into the high-G wall.

12. A method according to claim 11 wherein the biological fluid is whole blood.

13. A method according to claim 11 wherein said at least one higher density component is red blood cells.

14. A method for centrifugally separating a biological fluid into at least one higher density component and at least one lower density component, which includes plasma, the method comprising the steps of:

rotating a separation zone about a rotational axis, the rotating separation zone having radially spaced apart walls with a high-G side and a low-G side located closer to the rotational axis than the high-G side, the spaced apart walls defining a blood flow path that extends circumferentially about the rotational axis, the rotating separation zone including an entry region where the biological fluid enters the rotating separation zone to begin separation and a terminal wall that is circumferentially spaced from the entry region,

flowing the biological fluid into this rotating separation zone through a first path adjacent the entry region, to begin separation of the biological fluid into said at least one higher density component toward the high-G side and said at least one lower density component toward the low-G side,

flowing said at least one higher density component separated in the separation zone in a circumferential flow direction toward the terminal wall, and

removing said at least one higher density component from the rotating separation zone through a second path that communicates with the separation zone adjacent the terminal wall such that a substantial amount of said at least one lower density component cannot flow circumferentially past or into the second path, the second path including a portion that is recessed into the high-G wall.

15. A method according to claim 14 wherein the biological fluid is whole blood.

16. A method according to claim 14 wherein said at least one higher density component is red blood cells.