Assay and method for analyte sensing by detecting efficiency of radiation conversion
First Claim
1. An analyte sensing device for sensing a concentration of analyte in a fluid, the analyte sensing device comprising:
- a housing; and
an analyte sensing component disposed within the housing and including;
a first radiation converting component to convert radiation of a first wavelength to radiation having at least one different wavelength by receiving radiation of the first wavelength and, in response thereto, emitting radiation having at least one wavelength that is different from the first wavelength, wherein an efficiency of conversion by the first radiation converting component to radiation having at least one wavelength that is different from the first wavelength is dependent on the concentration of the analyte in the housing;
a second radiation converting component to convert radiation of a second wavelength to radiation having at least one wavelength that is different from the second wavelength by receiving radiation of the second wavelength and, in response thereto, emitting radiation having at least one wavelength that is different from the second wavelength, wherein an efficiency of conversion of the radiation of the second wavelength by the second radiation converting component is independent or substantially independent of the concentration of the analyte in the housing;
a plurality of beads or particles, wherein the second radiation converting component is embedded in or attached to at least one of the plurality of beads or particles;
an analyte-specific binding ligand; and
a macroporous matrix wherein (i) the analyte-specific binding ligand is attached to the macroporous matrix, and (ii) the plurality of beads or particles is embedded in the macroporous matrix.
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Accused Products
Abstract
In one aspect, the present invention is a system, a device and a method for sensing the concentration of an analyte in a fluid or matrix. The analyte may be glucose or any other chemical of interest. The fluid or matrix may be, for example, the fluid in a bioreactor, a food or agricultural product, any fluid or matrix in the body of an animal, or any other fluid or matrix whose concentration of an analyte is under investigation. In one embodiment, the analyte sensing device includes a housing having an interior space. Contained within the housing and in the interior space is one or more analyte sensing component(s). The analyte sensing component, in one embodiment, includes one or more radiation converting element(s), for example, converting chromophores. The radiation converting element(s) are capable of converting or modifying radiation of one or more wavelengths into radiation of one or more different wavelengths. The conversion efficiency of the radiation converting element(s) is at least partially dependent on the local concentration of an analyte within the analyte sensing component.
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Citations
63 Claims
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1. An analyte sensing device for sensing a concentration of analyte in a fluid, the analyte sensing device comprising:
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a housing; and an analyte sensing component disposed within the housing and including; a first radiation converting component to convert radiation of a first wavelength to radiation having at least one different wavelength by receiving radiation of the first wavelength and, in response thereto, emitting radiation having at least one wavelength that is different from the first wavelength, wherein an efficiency of conversion by the first radiation converting component to radiation having at least one wavelength that is different from the first wavelength is dependent on the concentration of the analyte in the housing; a second radiation converting component to convert radiation of a second wavelength to radiation having at least one wavelength that is different from the second wavelength by receiving radiation of the second wavelength and, in response thereto, emitting radiation having at least one wavelength that is different from the second wavelength, wherein an efficiency of conversion of the radiation of the second wavelength by the second radiation converting component is independent or substantially independent of the concentration of the analyte in the housing; a plurality of beads or particles, wherein the second radiation converting component is embedded in or attached to at least one of the plurality of beads or particles; an analyte-specific binding ligand; and a macroporous matrix wherein (i) the analyte-specific binding ligand is attached to the macroporous matrix, and (ii) the plurality of beads or particles is embedded in the macroporous matrix. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 60)
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32. An analyte sensing system for sensing a concentration of analyte in a fluid, the analyte sensing system comprising:
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an analyte sensing device including; a housing; and an analyte sensing component disposed within the housing and including; a first radiation converting component to convert radiation of a first wavelength to radiation having at least one different wavelength by receiving radiation of the first wavelength and, in response thereto, emitting radiation having at least one wavelength that is different from the first wavelength, wherein an efficiency of conversion by the first radiation converting component to radiation having at least one wavelength that is different from the first wavelength is dependent on the concentration of the analyte in the housing; a second radiation converting component to convert radiation of a second wavelength to radiation having at least one wavelength that is different from the second wavelength by receiving radiation of the second wavelength and, in response thereto, emitting radiation having at least one wavelength that is different from the second wavelength, wherein an efficiency of conversion of the radiation of the second wavelength by the second radiation converting component is independent or substantially independent of the concentration of the analyte in the housing; a plurality of beads or particles, wherein the second radiation converting component is embedded in or attached to at least one of the plurality of beads or particles; an analyte-specific binding ligand; and a macroporous matrix wherein (i) the analyte-specific binding ligand is attached to the the macroporous matrix, and (ii) the plurality of beads or particles is embedded in the macroporous matrix; a radiation providing unit to provide radiation at the first wavelength; and a radiation detecting unit to detect radiation having one or more wavelengths and to output data which is representative of the intensity of the radiation emitted by the first and second radiation converting components. - View Dependent Claims (33, 34, 35, 36, 37, 38, 39, 40, 41, 61, 62, 63)
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42. An analyte sensing device for sensing a concentration of analyte in a fluid, the analyte sensing device comprising:
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a housing; an analyte-analogue disposed within the housing; a macroporous matrix disposed within the housing; a plurality of beads or particles disposed or embedded in the macroporous matrix; an analyte-specific binding ligand, (i) attached to the macroporous matrix, and capable of binding to analyte and/or analyte-analogue; a first radiation converting component, attached to the analyte-analogue, to convert radiation of a first wavelength to radiation having at least one different wavelength by receiving radiation of the first wavelength and, in response thereto, emitting radiation having at least one wavelength that is different from the first wavelength, wherein an efficiency of conversion by first radiation converting component to radiation having at least one wavelength that is different from the first wavelength is dependent on the concentration of analyte in the housing; a second radiation converting component to convert radiation of a second wavelength to radiation having at least one wavelength that is different from the second wavelength by receiving radiation of the second wavelength and, in response thereto, emitting radiation having at least one wavelength that is different from the second wavelength, wherein an efficiency of conversion of the radiation of the second wavelength by the second radiation converting component is independent or substantially independent of the concentration of the analyte in the housing, wherein the second radiation converting component is embedded in or attached to at least one of the plurality of beads or particles; and a radiation absorbing component, disposed within the housing, having a proximity to the analyte-specific binding ligand that is sufficient to alter the efficiency of the conversion of the radiation of the first wavelength by the first radiation converting component. - View Dependent Claims (43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59)
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Specification