High throughput separations based analysis systems
First Claim
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1. A method of separating one or more sample materials into a plurality of fractions, comprising;
- providing a system comprising;
a separation channel having a separation matrix disposed therein,a source of additional separation matrix in fluid communication with the separation channel,an injection channel in fluid communication with the separation channel at an intermediate point along the injection channel, anda sample loading channel in fluid communication with the injection channel, a source of a first sample material, and a source of a reagent;
transporting the first sample material and the reagent into the sample loading channel, wherein the first sample material and the reagent form a mixture;
injecting a portion of the mixture from the sample loading channel, through the injection channel, into the separation channel;
separating the first sample material within the mixture into a plurality of fractions;
displacing a portion of the separation matrix from the separation channel after separating the first sample material into a plurality of fractions; and
transporting a second sample material into the sample loading channel, wherein the force used to transport the second sample material into the sample loading channel displaces the portion of the separation matrix from the separation channel and replaces the displaced portion of the separation matrix with additional separation matrix.
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Abstract
Devices, systems and methods for use in separating sample materials into different fractions that employ bulk fluid flow for loading of samples followed by electrophoretic separation of the sample material. Devices employ configurations that optionally allow bulk sample loading with some or no displacement of a separation matrix within a separation conduit. Methods of using these devices, and systems that incorporate these devices are also envisioned.
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Citations
27 Claims
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1. A method of separating one or more sample materials into a plurality of fractions, comprising;
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providing a system comprising; a separation channel having a separation matrix disposed therein, a source of additional separation matrix in fluid communication with the separation channel, an injection channel in fluid communication with the separation channel at an intermediate point along the injection channel, and a sample loading channel in fluid communication with the injection channel, a source of a first sample material, and a source of a reagent; transporting the first sample material and the reagent into the sample loading channel, wherein the first sample material and the reagent form a mixture; injecting a portion of the mixture from the sample loading channel, through the injection channel, into the separation channel; separating the first sample material within the mixture into a plurality of fractions; displacing a portion of the separation matrix from the separation channel after separating the first sample material into a plurality of fractions; and transporting a second sample material into the sample loading channel, wherein the force used to transport the second sample material into the sample loading channel displaces the portion of the separation matrix from the separation channel and replaces the displaced portion of the separation matrix with additional separation matrix. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27)
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Specification