Apparatus enabling liquid transfer by capillary action therein

• US 7,169,353 B1
• Filed: 03/09/2000
• Issued: 01/30/2007
• Est. Priority Date: 03/09/1999
• Status: Expired due to Fees

• Current Assignee: Biomerieux SA (Compagnie Merieux Alliance SAS)
• Original Assignee: Biomerieux SA (Compagnie Merieux Alliance SAS)
• Inventors: Colin, Bruno, Privat, Marie
• Primary Examiner(s): WARDEN, JILL ALICE
• Application Number:
  US09/936,077
• Time in Patent Office: 2,518 Days
• Field of Search: 422/102, 422 56- 58, 422/100
• US Class Current: 422/412
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/089 Virtual walls for guiding liquids
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 3/502792 for moving individual droplets on a plate, e.g. by locally altering surface tension

Abstract

An apparatus (1) which includes at least one planar surface (2) having compartments (3) which are defined by a partition (4), the compartments creating a space which makes it possible to displace at least one liquid sample (5 and/or 15) and, when there are at least two liquid samples (5 and 15), makes it possible to displace them both in an independent way and bring them together so that they can react with one another. The compartments (3) include at least two different types of groove: a deep groove (6), capable of partitioning the sample(s) (5 and/or 15), and a shallow groove capable of receiving the sample (s) (5 and/or 15), the two types of groove (6 and 16) making it possible to direct sample movements (5 and/or 15) by altering the orientation of the apparatus (1). The invention is particularly applicable for the micromanipulation of fluids in biological applications.

Claims

1. A test card comprising at least one planar surface wherein at least two compartments are made and defined by a partition comprising a film, the compartments creating a space which makes it possible to displace at least two liquid samples independently of one another, the compartments comprising at least two different types of grooves:

at least one deep groove capable of partitioning samples from one another, the depth and the width of the deep groove in relation to the partition being such that capillary action of a sample is not enabled, and

at least two shallow grooves, each of the shallow grooves being capable of receiving one of the samples, respectively, the depth of shallow grooves in relation to the partition being such that capillary action of the sample is enabled, each shallow groove being adjacent to the deep groove, along the entire length of said deep groove, wherein said test card is adapted for analysis of chemical, biochemical or biological analytes.

2. The test card of claim 1, wherein a deep groove is positioned between two shallow grooves.

3. The test card of claim 2, wherein the two shallow grooves meet at one of the ends of the deep groove to create a reaction zone there, where the two liquid samples may be brought together.

4. The test card of claim 3, wherein the distance between the reaction zone and the partition is such that capillary action is enabled.

5. The test card of claim 3, wherein the distance between the reaction zone and the partition is such that capillary action is not enabled.