Universal nucleotides for nucleic acid analysis
First Claim
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1. A kit for sequencing a target nucleic acid template, comprising at least one universal nucleotide, at least one polymerase, and at least one specific terminator;
- wherein one or more of the at least one universal nucleotides is individually selected from 2′
-deoxy-7-azaindole-5′
-triphosphate (d7AITP), 2′
-deoxy-isocarbostyril-5′
-triphosphate (dICSTP), 2′
-deoxy-propynylisocarbostyril-5′
-triphosphate (dPICSTP), 2′
-deoxy-6-methyl-7-azaindole-5′
-triphosphate (dM7AITP), 2′
-deoxy-imidizopyridine-5′
-triphosphate (dImPyTp), 2′
-deoxy-pyrrollpyrizine-5′
-triphosphate (dPPTP), 2′
-deoxy-allenyl-7-azaindole-5′
-triphosphate (dA7AITP), and 2′
-deoxy-propynyl-7-azaindole-5′
-triphosphate (dP7AITP).
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Abstract
The invention includes methods and kits for making and analyzing primer extension products incorporating one or more universal bases, including methods and kits for nucleic acid sequencing and microsatellite analysis.
64 Citations
10 Claims
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1. A kit for sequencing a target nucleic acid template, comprising at least one universal nucleotide, at least one polymerase, and at least one specific terminator;
- wherein one or more of the at least one universal nucleotides is individually selected from 2′
-deoxy-7-azaindole-5′
-triphosphate (d7AITP), 2′
-deoxy-isocarbostyril-5′
-triphosphate (dICSTP), 2′
-deoxy-propynylisocarbostyril-5′
-triphosphate (dPICSTP), 2′
-deoxy-6-methyl-7-azaindole-5′
-triphosphate (dM7AITP), 2′
-deoxy-imidizopyridine-5′
-triphosphate (dImPyTp), 2′
-deoxy-pyrrollpyrizine-5′
-triphosphate (dPPTP), 2′
-deoxy-allenyl-7-azaindole-5′
-triphosphate (dA7AITP), and 2′
-deoxy-propynyl-7-azaindole-5′
-triphosphate (dP7AITP). - View Dependent Claims (2, 3, 4, 5)
- wherein one or more of the at least one universal nucleotides is individually selected from 2′
-
6. A kit for detecting a short tandem repeat in a target nucleic acid template, comprising at least one universal nucleotide, at least one polymerase, and at least one primer comprising a sequence that is complementary to a sequence adjacent to a short tandem repeat in the target nucleic acid template;
- wherein one or more of the at least one universal nucleotides is individually selected from 2′
-deoxy-7-azaindole-5′
-triphosphate (d7AITP), 2′
-deoxy-isocarbostyril-5′
-triphosphate (dICSTP), 2′
-deoxy-propynylisocarbostyril-5′
-triphosphate (dPICSTP), 2′
-deoxy-6-methyl-7-azaindole-5′
-triphosphate (dM7AITP), 2′
-deoxy-imidizopyridine-5′
-triphosphate (dImPyTp), 2′
-deoxy-pyrrollpyrizine-5′
-triphosphate (dPPTP), 2′
-deoxy-allenyl-7-azaindole-5′
-triphosphate (dA7AITP), and 2′
-deoxy-propynyl-7-azaindole-5′
-triphosphate (dP7AITP). - View Dependent Claims (7, 8, 9, 10)
- wherein one or more of the at least one universal nucleotides is individually selected from 2′
Specification