Bone marrow cell differentiation

• US 7,169,608 B2
• Filed: 10/17/2003
• Issued: 01/30/2007
• Est. Priority Date: 10/18/2002
• Status: Expired due to Fees

• Current Assignee: University of Florida Research Foundation, Incorporated (State University System of Florida)
• Original Assignee: University of Florida Research Foundation, Incorporated (State University System of Florida)
• Inventors: Petersen, Bryon E., Oh, Seh-hoon
• Primary Examiner(s): Afremova; Vera
• Application Number:
  US10/687,674
• Publication Number:
  US 20040157325A1
• Time in Patent Office: 1,201 Days
• Field of Search: 435/377, 435/372, 435/375
• US Class Current: 435/377
• CPC Class Codes: A61P 3/10 for hyperglycaemia, e.g. antidiabetics
• CPC Class Codes: C12N 2500/30 Organic components metal chelators C12N2500/10; calcitonin C12N2500/14; transferrin C12N2500/24
• CPC Class Codes: C12N 2500/34 Sugars
• CPC Class Codes: C12N 2500/62 DMSO
• CPC Class Codes: C12N 2506/11 from blood or immune system cells
• CPC Class Codes: C12N 2506/1353 from bone marrow mesenchymal stem cells (BM-MSC)
• CPC Class Codes: C12N 5/0676 Pancreatic cells

Abstract

Bone marrow cells are induced to differentiate into pancreatic hormone-producing cells in vitro and to repopulate a pancreas in vivo. These insulin-producing cells can be used to regenerate a damaged pancreas and reverse hyperglycemia in mammals.

Claims

1. A method of differentiating a mammalian bone marrow cell into an pancreatic hormone-producing cell, the method comprising the steps of:

(A) providing the hone marrow cell;

(B) first culturing the bone marrow cell in a low-glucose serum free medium comprising about 5.5 mM glucose and 1% DMSO for at least 3 days; and

(C) then culturing the bone marrow cell in a high-glucose medium comprising about 25 mM glucose and 10% serum under appropriate conditions and for at least 7 days to promote differentiation of the cell into an pancreatic hormone-producing cell.

2. The method of claim 1, wherein the bone marrow cell is a rodent cell.

3. The method of claim 2, wherein the rodent cell is a rat cell.

4. The method of claim 1, wherein the pancreatic hormone-producing cell produces insulin.

5. The method of claim 1, wherein the pancreatic hormone-producing cell produces glucagon.

6. The method of claim 1, wherein the pancreatic hormone-producing cell produces somatostatin.

7. The method of claim 1, wherein the pancreatic hormone-producing cell produces pancreatic polypeptide.

8. The method of claim 1, wherein the high-glucose medium comprises DMEM and fetal bovine serum.