Inactivated enzyme variants and associated process and reagent system
First Claim
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1. A glucose dehydrogenase variant having about 3% or less residual enzymatic activity relative to the wild type enzyme and which retains about 20% or more of the coenzyme binding activity of the wild type enzyme, wherein said variant comprises an amino acid substitution at position 145 or 158 of the amino acid sequence of SEQ ID NO:
- 6 of B. subtilis.
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Abstract
The present invention relates to oxidoreductase apoenzyme variants which are enzymatically inactive but have coenzyme-binding properties. Further, the present invention relates to DNA sequences encoding these oxidoreductase apoenzyme variants, expression vectors containing such DNA sequences and the use of these oxidoreductase apoenzyme variants in diagnostic applications.
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9 Claims
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1. A glucose dehydrogenase variant having about 3% or less residual enzymatic activity relative to the wild type enzyme and which retains about 20% or more of the coenzyme binding activity of the wild type enzyme, wherein said variant comprises an amino acid substitution at position 145 or 158 of the amino acid sequence of SEQ ID NO:
- 6 of B. subtilis.
- View Dependent Claims (2, 3, 4, 5)
- 6. A glucose dehydrogenase variant comprising the amino acid sequence as shown in SEQ ID NO:
- 8. A glucose dehydrogenase variant comprising the amino acid sequence as shown in SEQ ID NO:
Specification