Integrated portable biological detection system
First Claim
1. An integrated system for the analysis of eukaroytic and/or prokaryotic cells in a biological sample comprising:
- an electronic system for cell separation, cell lysis, sample preparation, and sample analysis further comprising a flow cell, an input port coupled to the flow cell, an output port coupled to the flow cell, a plurality of individually addressable electrodes positioned within the flow cell and coupled to a power source configured to electronically disrupt cell membranes within the sample, and an array of probes coupled to the electrodes and adapted to bind to predetermined components within the sample for analysis of the components, and a heating element coupled to the flow cell, wherein the flow cell is adapted to receive the sample via the input;
a detector operatively positioned to detect the molecules bound to the probes by a detectable signal;
a power source coupled to the flow cell; and
a portable housing configured to contain the electronic system, the detector, and the power source.
4 Assignments
0 Petitions
Accused Products
Abstract
We have performed separation of bacterial and cancer cells from peripheral human blood in microfabricated electronic chips by dielectrophoresis. The isolated cells were examined by staining the nuclei with fluorescent dye followed by laser induced fluorescence imaging. We have also released DNA and RNA from the isolated cells electronically and detected specific marker sequences by DNA amplification followed by electronic hybridization to immobilized capture probes. Efforts towards the construction of a “laboratory-on-a-chip” system are presented which involves the selection of DNA probes, dyes, reagents and prototyping of the fully integrated portable instrument.
-
Citations
23 Claims
-
1. An integrated system for the analysis of eukaroytic and/or prokaryotic cells in a biological sample comprising:
-
an electronic system for cell separation, cell lysis, sample preparation, and sample analysis further comprising a flow cell, an input port coupled to the flow cell, an output port coupled to the flow cell, a plurality of individually addressable electrodes positioned within the flow cell and coupled to a power source configured to electronically disrupt cell membranes within the sample, and an array of probes coupled to the electrodes and adapted to bind to predetermined components within the sample for analysis of the components, and a heating element coupled to the flow cell, wherein the flow cell is adapted to receive the sample via the input; a detector operatively positioned to detect the molecules bound to the probes by a detectable signal; a power source coupled to the flow cell; and a portable housing configured to contain the electronic system, the detector, and the power source. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
-
-
11. A method for performing an analysis of a biological sample having desired cells and undesired cells using an integrated portable system comprising a flow cell, a plurality of individually addressable electrodes positioned within the flow cell, and a portable housing containing the flow cell, the method comprising:
-
introducing the sample into the flow cell via an input port coupled to the flow cell; creating a dielectric force pattern by individually biasing the electrodes positioned within the flow cell; subjecting the sample to the dielectric force pattern to separate the undesired cells from the desired cells in the sample; isolating the desired cells by maintaining an attractive bias for the desired cells and introducing a flow of wash buffer through the flow cell via the input port to eliminate the undesired cells, wherein the undesired cells are removed from the flow cell via an output port coupled to the flow cell; lysing the desired cells by applying an electric force within the flow cell to electronically disrupt the desired cells into a plurality of components; and analyzing the components of the desired cells by hybridizing the components with probes to form probe-target hybrids within the flow cell, and detecting the probe-target hybrids while the hybrids are within the flow cell, wherein the probes are coupled to the electrodes. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22)
-
-
23. A method for performing an analysis of a biological sample having desired cells and undesired cells using an integrated portable system, the method comprising:
-
providing an integrated portable system comprising an electronic system having a flow cell, a plurality of individually addressable electrodes positioned within the flow cell, a plurality of probes coupled to the electrodes, a detector configured to detect the probes, a power source coupled to the flow cell, and a portable housing containing the electronic system, the detector, and the power source; introducing the sample into the integrated portable system by injecting the sample into the flow cell via an input port coupled to the flow cell, wherein the input port is accessed through the portable housing; creating a dielectric force pattern by individually biasing the electrodes positioned within the flow cell; subjecting the sample to the dielectric force pattern to separate the undesired cells from the desired cells in the sample; isolating the desired cells by maintaining an attractive bias for the desired cells and introducing a flow of wash buffer through the flow cell via the input port to eliminate the undesired cells, wherein the undesired cells are removed from the flow cell via an output port coupled to the flow cell; lysing the desired cells by applying an electric force within the flow cell to electronically disrupt the desired cells into a plurality of components; and analyzing the components of the desired cells by hybridizing the components with probes to form probe-target hybrids within the flow cell, and detecting the probe-target hybrids while the hybrids are within the flow cell.
-
Specification