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System and method for temperature gradient capillary electrophoresis

  • US 7,175,750 B2
  • Filed: 11/05/2002
  • Issued: 02/13/2007
  • Est. Priority Date: 09/01/2000
  • Status: Expired due to Fees
First Claim
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1. A temperature gradient electrophoresis-based method for generating data indicative of the presence of a single nucleotide polymorphism or a mutation in a biological sample, comprising:

  • providing an unknown double-stranded polynucleotide sample;

    subjecting the unknown double-stranded polynucleotide sample to temperature gradient gel electrophoresis, the unknown double-stranded polynucleotide sample including non-desalted polymerase chain reaction (PCR) products;

    subjecting a reference double-stranded polynucleotide to temperature gradient electrophoresis;

    irradiating the unknown double-stranded polynucleotide with light to generate a first spectroscopic signal,irradiating the reference double-stranded polynucleotide with light to generate a second spectroscopic signal;

    converting the first and second spectroscopic signals into first and second data; and

    determining the presence of a single nucleotide polymorphism or a mutation in the unknown double-stranded polynucleotide sample based on the first and second data.

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