Method of making a plastic colorimetric resonant biosensor device with liquid handling capabilities

• US 7,175,980 B2
• Filed: 07/23/2002
• Issued: 02/13/2007
• Est. Priority Date: 10/30/2000
• Status: Expired due to Fees

• Current Assignee: X-Body Incorporated (Bristol-Myers Squibb Company)
• Original Assignee: Sru Biosystems Incorporated
• Inventors: Li, Peter, Cunningham, Brian, Qiu, Jean
• Primary Examiner(s): Chin; Christopher L.
• Application Number:
  US10/201,878
• Publication Number:
  US 20030026891A1
• Time in Patent Office: 1,666 Days
• Field of Search: None
• US Class Current: 435/4
• CPC Class Codes: B01L 2200/0689 Sealing
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0819 Microarrays; Biochips
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: G01N 21/253 for batch operation, i.e. multisample apparatus analytical automats G01N35/00
• CPC Class Codes: G01N 21/4788 Diffraction for sizing particles G01N15/0205
• CPC Class Codes: G01N 21/7743 the reagent-coated grating coupling light in or out of the waveguide
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: G02B 21/34 Microscope slides, e.g. mounting specimens on microscope slides
• CPC Class Codes: G02B 5/1809 with pitch less than or comparable to the wavelength
• CPC Class Codes: Y10T 156/10 Methods of surface bonding and/or assembly therefor

Abstract

Methods of producing liquid handling biosensor devices are provided. The liquid handling biosensor devices allow detection of biomolecular interactions in liquid. The use of labels is not required and the methods can be performed in a high-throughput manner.

Claims

1. A method of making a liquid handling colorimetric resonant reflection biosensor device, comprising:

(a) die cutting a transfer pressure sensitive adhesive into a pattern of a liquid holding part to create a patterned adhesive;

(b) transferring the patterned adhesive onto the liquid holding part;

(c) contacting a biosensor with the patterned adhesive of the liquid holding part, and

(d) applying pressure to the biosensor or liquid holding part;

whereby a liquid handling colorimetric resonant reflection biosensor device is made.

2. The method of claim 1, wherein the biosensor comprises a grating comprised of or coated with a dielectric material having a high refractive index or a reflective material, wherein when the biosensor is illuminated light is resonantly reflected.

3. The method of claim 1, wherein the liquid holding part is a bottomless microliter plate or a fluid flow channel.