BMP-11 compositions

• US 7,175,997 B2
• Filed: 07/05/2002
• Issued: 02/13/2007
• Est. Priority Date: 05/12/1993
• Status: Expired due to Fees

• Current Assignee: Genetics Institute LLC (Pfizer Inc.)
• Original Assignee: Genetics Institute LLC (Pfizer Inc.)
• Inventors: Wozney, John M., Celeste, Anthony J.
• Primary Examiner(s): Romeo; David S.
• Application Number:
  US10/188,886
• Publication Number:
  US 20030166910A1
• Time in Patent Office: 1,684 Days
• Field of Search: 536/23.5, 530/350
• US Class Current: 435/69.1
• CPC Class Codes: A61K 38/1875 Bone morphogenic factor; Osteogenins; Osteogenic factor; Bone-inducing factor
• CPC Class Codes: A61P 13/02 of urine or of the urinary tract, e.g. urine acidifiers
• CPC Class Codes: A61P 15/00 Drugs for genital or sexual disorders for disorders of sex hormones A61P5/24; Contraceptives
• CPC Class Codes: A61P 15/18 Feminine contraceptives
• CPC Class Codes: A61P 19/00 Drugs for skeletal disorders
• CPC Class Codes: A61P 25/00 Drugs for disorders of the nervous system
• CPC Class Codes: A61P 25/28 for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
• CPC Class Codes: A61P 35/00 Antineoplastic agents
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: A61P 7/00 Drugs for disorders of the blood or the extracellular fluid
• CPC Class Codes: C07K 14/51 Bone morphogenetic factor; Osteogenins; Osteogenic factor; Bone-inducing factor
• CPC Class Codes: C07K 14/575 Hormones derived from pro-opiomelanocortin, pro-enkephalin or pro-dynorphin C07K14/665, e.g. corticotropin C07K14/695
• CPC Class Codes: C07K 2319/00 Fusion polypeptide
• CPC Class Codes: C12N 2501/155 Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
• CPC Class Codes: C12N 5/0619 Neurons
• CPC Class Codes: Y10S 930/12 Growth hormone, growth factor other than t-cell or b-cell growth factor, and growth hormone releasing factor; related peptides

Abstract

Purified BMP-11 proteins and processes for producing them are disclosed. Recombinant DNA molecules encoding the BMP-11 proteins are also disclosed. The proteins may be useful in regulating follicle stimulating hormone, such as for contraception. In addition, the proteins may be useful for the induction of bone, cartilage and/or other connective tissue.

Claims

1. An isolated DNA sequence comprising a DNA sequence chosen from:

(a) nucleotides 1 to 759 of SEQ ID NO;

10; and

(b) nucleotides encoding amino acids -253 to -1 of SEQ ID NO;

11.

3. A vector comprising the DNA molecule of claim 1.

4. A host cell transformed with a vector of claim 3.

5. The host cell of claim 4, wherein the host cell is chosen from a mammalian cell, a bacterial cell, a yeast cell, and an insect cell.

6. The host cell of claim 5, wherein the host cell is a mammalian cell.

7. The host cell of claim 6 wherein the mammalian cell is chosen from a CHO, a COS-1, and a CV-1 cell.

8. The host cell of claim 6, wherein the mammalian cell is a CHO cell.

9. The host cell of claim 5, wherein the host cell is a bacterial cell chosen from an E. coli cell, a B. subtilis cell, and a Pseudomonas cell.

10. A method for producing a polypeptide comprising:

(a) culturing a host cell transformed with a DNA molecule of claim 1; and

(b) recovering and purifying the polypeptide encoded by the DNA molecule of claim 1 from the culture medium.

11. The method of claim 10, wherein the host cell is transformed with a DNA molecule comprising:

(a) nucleotides 1 to 759 of SEQ ID NO;

010 or

(b) equivalent degenerative codon sequences of (a).

2. An isolated DNA sequence consisting of a DNA sequence chosen from:

(a) nucleotides 1 to 759 of SEQ ID NO;

10; and

(b) nucleotides encoding amino acids -253 to -1 of SEQ ID NO;

11.