Solid culture medium and method for preparing the same

• US 7,176,014 B2
• Filed: 08/09/2001
• Issued: 02/13/2007
• Est. Priority Date: 01/29/2001
• Status: Expired due to Fees

• Current Assignee: Morinaga Milk Industry Company Limited
• Original Assignee: Morinaga Milk Industry Company Limited
• Inventors: Fukuwatari, Yasuo, Nakagawa, Minoru, Sotoyama, Kazuyoshi, Kiyotaki, Kenji, Yano, Yoichiro, Karino, Kenichiro, Sasaki, Kazue
• Primary Examiner(s): Lankford, Jr.; Leon B.
• Application Number:
  US10/432,089
• Publication Number:
  US 20040041123A1
• Time in Patent Office: 2,014 Days
• Field of Search: 435/252.1, 435/256.8, 435/404, 435/253.6
• US Class Current: 435/253.6
• CPC Class Codes: C12N 1/00 Microorganisms, e.g. protozoa; Compositions thereof medicinal preparations containing material from protozoa, bacteria or viruses A61K35/66, from algae A61K36/02, from fungi A61K36/06; preparing medicinal bacterial antigen or antibody compositions, e.g. bacterial vaccines, A61K39/00; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
• CPC Class Codes: C12N 1/20 Bacteria; Culture media therefor
• CPC Class Codes: G01N 2333/31 from Staphylococcus (G)
• CPC Class Codes: G01N 2333/32 from Bacillus (G)
• CPC Class Codes: G01N 33/56911 Bacteria
• CPC Class Codes: G01N 33/56938 Staphylococcus

Abstract

A solid medium having a 10 minute-average water absorption rate of at least 0.05 ml/minute, which is obtainable by a method for producing a solid medium comprising the steps of dissolving components of the solid medium other than solvent water into the solvent water, solidifying the obtained solution, and drying the solidified medium to remove water, wherein water is removed in such an amount that the solid medium after the removal of water should have the 10 minute-average water absorption rate of at least 0.05 ml/minute, and the amount of the solvent water is larger than a prescribed amount by an amount almost equal to the amount of the water to be removed. The solid medium does not cause growth inhibition of microorganisms due to drying, shows a superior water absorption rate to enable application of a larger amount of a sample in a short period of time, and is suitable for quick and accurate measurement tests of microbial numbers.

Claims

1. A solid agar medium for microorganisms having a water content of at least 90%, and having a 10 minute-average water absorption rate of at least 0.05 ml/minute, which is obtainable by a method comprising the steps of:

dissolving components of the solid medium other than solvent water into the solvent water,

solidifying the obtained solution, and

drying the solidified medium to remove water,

wherein the 10-minute-average water absorption rate is calculated for an area of the medium which is about 58.1 cm² with a weight of about 15 gm, and wherein the amount of the solvent water is larger than a prescribed amount by an amount almost equal to the amount of the water to be removed.

2. The solid agar medium according to claim 1, wherein the amount of the removed water is at least 5% of the solvent water.

3. The solid agar medium according to claim 1, wherein the amount of the removed water to be removed is at least 30% of the solvent water.

4. A method for producing a solid agar medium for microorganisms which comprises the steps of:

dissolving components of the solid agar medium into a solvent water,

solidifying the obtained solution, and

drying the solidified medium to remove water,

wherein water is removed in such an amount that the solid medium after the removal of water should have a water content of at least 90%, and a 10 minute-average water absorption rate of at least 0.05 ml/minute calculated for an area of the medium which is about 58.1 cm² with a weight of about 15 gm, and wherein the amount of solvent water is larger than the prescribed amount by an amount almost equal to the amount of the removed water.

5. The method according to claim 4, wherein the amount of the removed water is at least 5% of the solvent water.

6. The method according to claim 4, wherein the amount of the removed water is at least 30% of the solvent water.