Endothelial cells derived from primate embryonic stem cells
First Claim
1. A method for directing the differentiation of primate embryonic stem cells into cells of the endothelial lineage comprising the steps of(a) culturing a culture of embryonic stem cells in a medium containing vascular endothelial cell growth factor, basic fibroblast growth factor, insulin-like growth factor and epidermal growth factor;
- and(b) sub-culturing the cells which have the morphology of endothelial cells to obtain a culture composed of cells over 90% of which are of endothelial lineage as determined by morphology.
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Abstract
A method is described to induce primate embryonic stem cells to differentiate into a relatively homogenous population of endothelial cells. The ES derived endothelial cells have the general morphological and cell surface marker characteristics of endothelial cells. The ES derived endothelial cells also are capable of inducing and participating in blood vessel formation (or vascularization) when transplanted into tissue in vivo.
15 Citations
4 Claims
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1. A method for directing the differentiation of primate embryonic stem cells into cells of the endothelial lineage comprising the steps of
(a) culturing a culture of embryonic stem cells in a medium containing vascular endothelial cell growth factor, basic fibroblast growth factor, insulin-like growth factor and epidermal growth factor; - and
(b) sub-culturing the cells which have the morphology of endothelial cells to obtain a culture composed of cells over 90% of which are of endothelial lineage as determined by morphology. - View Dependent Claims (2, 3, 4)
- and
Specification