Method for detecting a lipoprotein-acute phase protein complex and predicting an increased risk of system failure or mortality
First Claim
1. A method for testing the effectiveness of a therapeutic for treatment of hemostatic dysfunction, comprising the steps of (a) providing a test sample from a patient with hemostatic dysfunction, (b) adding a divalent metal cation reagent to the test sample wherein the reagent does not cause fibrin formation, (c) measuring the formation of a complex comprising C reactive protein (CRP) and at least one human lipoprotein selected from the group consisting of very low density lipoprotein (VLDL) and intermediate density lipoprotein (IDL), (d) administering a therapeutic to said patient, (e) repeating the steps (a) through (d) at a later time, wherein a decrease in said complex formation at said later time is indicative of the efficacy of said therapeutic for treatment of hemostatic dysfunction.
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Abstract
A method for diagnosing a condition of a patient involves the steps of (a) adding one or more reagents to a test sample from a patient, the test samples comprising at least part of a blood sample from the patient, in order to cause formation of a complex comprising at least one acute phase protein and at least one human lipoprotein, while causing substantially no fiber polymerization; (b) measuring the formation of the complex over time so as to derive a time-dependent measurement profile, and (c) determining a slope and/or total change in the time-dependent measurement profile, so as to diagnose a condition of the patient. A greater formation of the complex is correlated to increased probability of death of the patient.
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Citations
5 Claims
- 1. A method for testing the effectiveness of a therapeutic for treatment of hemostatic dysfunction, comprising the steps of (a) providing a test sample from a patient with hemostatic dysfunction, (b) adding a divalent metal cation reagent to the test sample wherein the reagent does not cause fibrin formation, (c) measuring the formation of a complex comprising C reactive protein (CRP) and at least one human lipoprotein selected from the group consisting of very low density lipoprotein (VLDL) and intermediate density lipoprotein (IDL), (d) administering a therapeutic to said patient, (e) repeating the steps (a) through (d) at a later time, wherein a decrease in said complex formation at said later time is indicative of the efficacy of said therapeutic for treatment of hemostatic dysfunction.
Specification