Microarrays and their manufacture by slicing

• US 7,179,638 B2
• Filed: 01/25/2002
• Issued: 02/20/2007
• Est. Priority Date: 07/30/1999
• Status: Expired due to Fees

• Current Assignee: Large Scale Proteomics Corporation
• Original Assignee: Large Scale Biology Corporation
• Inventors: Anderson, N. Leigh, Anderson, Norman G., Braatz, James A.
• Primary Examiner(s): Chin; Christopher L.
• Application Number:
  US10/061,969
• Publication Number:
  US 20030044855A1
• Time in Patent Office: 1,852 Days
• Field of Search: 422/57, 422/58, 435/6, 435/287.1, 435/287.2, 436/518, 436/524, 436/525, 436/527
• US Class Current: 435/287.2
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00515 in the shape of strings
• CPC Class Codes: B01J 2219/0052 in the shape of elongated tubes
• CPC Class Codes: B01J 2219/00524 in the shape of fiber bundles
• CPC Class Codes: B01J 2219/00547 Bar codes
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/0061 The surface being organic
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00617 by chemical means
• CPC Class Codes: B01J 2219/00621 by physical means, e.g. trenches, raised areas
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/0063 Other, e.g. van der Waals forces, hydrogen bonding
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00641 the porous medium being continuous, e.g. porous oxide substrates
• CPC Class Codes: B01J 2219/00644 the porous medium being present in discrete locations, e.g. gel pads
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00664 Three-dimensional arrays
• CPC Class Codes: B01J 2219/00673 Slice arrays
• CPC Class Codes: B01J 2219/00707 : separated from the reactor apparatus
• CPC Class Codes: B01J 2219/0072 : Organic compounds
• CPC Class Codes: B01J 2219/00722 : Nucleotides
• CPC Class Codes: B01J 2219/00731 : Saccharides
• CPC Class Codes: B01L 3/50857 : using arrays or bundles of open capillaries for holding samples
• CPC Class Codes: C12Q 1/6837 : using probe arrays or probe chips C12Q1/6874 takes precedence
• CPC Class Codes: C40B 40/06 : Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 40/12 : Libraries containing saccharides or polysaccharides, or derivatives thereof
• CPC Class Codes: C40B 70/00 : Tags or labels specially adapted for combinatorial chemistry or libraries, e.g. fluorescent tags or bar codes
• CPC Class Codes: G01N 2500/00 : Screening for compounds of potential therapeutic value
• CPC Class Codes: G01N 33/54313 : the carrier being characterised by its particulate form
• CPC Class Codes: G01N 33/5436 : with ligand physically entrapped within the solid phase liposomes G01N33/5432; immunological test elements G01N33/54386
• CPC Class Codes: G01N 33/54366 : Apparatus specially adapted for solid-phase testing

Abstract

Microarrays are prepared by using a separate fiber for each compound being used in the microarray. The fibers are bundled and sectioned to form a thin microarray that may be glued to a backing.

Claims

1. A microarray comprising a solid phase support and at least about 500 cells per square centimeter wherein each cell contains an agent of interest that is not chemically bound to the solid phase support and a plurality of structures bound to said solid phase support, wherein each of a plurality of structures has an immobilized agent of interest available for binding to a target, wherein the target is a binding partner for the agent of interest, and wherein the microarray contains a plurality of different agents of interest in a corresponding plurality of different structures and, wherein the structures are permeable to the target.

2. A microarray of claim 1 comprising a solid phase support and at least about 500 cells per square centimeter wherein each cell contains an agent of interest which is a macromolecule, a microorganism, a plant or animal cell, an organelle or a fraction of a biological cell.

3. The microarray of claim 1 containing at least about 1,000 cells per square centimeter.

4. The microarray of claim 1 containing at least about 5,000 cells per square centimeter.

5. The microarray of claim 1, wherein the structures are a gel.

6. A microarray comprising a solid phase support and at least about 500 cells per square centimeter wherein each cell contains an agent of interest that is not chemically bound to the solid phase support and a plurality of structures bound to said solid phase support, wherein each of a plurality of structures has an immobilized agent of interest available for binding to a target, wherein the target is a binding partner for the agent of interest, and wherein the microarray contains a plurality of different agents of interest in a corresponding plurality of different structures, wherein each of the structure forms a hollow chamber with the agent of interest immobilized on an inner surface of the hollow chamber.

7. The microarray of claim 6 containing at least about 1,000 cells per square centimeter.

8. The microarray of claim 6 wherein the agent of interest is a macromolecule, a microorganism, a plant or animal cell, an organelle or a fraction of a biological cell.

9. A microarray comprising a solid phase support and at least about 500 cells per square centimeter wherein each cell contains an agent of interest that is not chemically bound to the solid phase support wherein the solid phase support has a flat surface, and a plurality of structures projecting away from the plane of the solid phase support, wherein each of the plurality of the structures has an agent of interest bound thereto, and wherein the microarray contains a plurality of different agents of interest in a corresponding plurality of different structures.

10. The microarray of claim 9 containing at least about 1,000 cells per square centimeter.

11. The microarray of claim 9 wherein the macromolecule is a protein.

12. The microarray of claim 9 wherein the agent of interest is synthesized prior to contacting the solid phase support.

13. The microarray of claim 9 wherein each cell is formed by a solid material adhered to said solid phase support and wherein each solid material contains an agent of interest.

14. The microarray of claim 9 wherein the structures are made of impermeable material and the agents of interest are immobilized on a surface thereof.

15. The microarray of claim 9 wherein the structures were formed by solid material between the structures being removed.

16. The microarray of claim 9 containing at least about 5,000 cells per square centimeter.

17. The microarray of claim 16 containing at least about 10,000 cells per square centimeter.

18. The microarray of claim 17 containing at least about 100,000 cells per square centimeter.

19. The microarray of claim 9 further comprising a hydrophobic material between the cells on the solid phase support.