Thermal strip thermocycler
First Claim
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1. A thermal strip thermocycler comprising:
- a substrate,a rectangular porous membrane, overlaying the substrate and enclosed by a clear impermeable non-electrically conductive material and having a first end and a second end opposing the first end,a conductive circuit printed on a surface of the substrate,the conductive circuit having parallel alternating zones of temperature produced by resistive heating elements positioned along the conductive circuit, and positioned such that a long axis and direction of flow within the porous membrane is perpendicular to the parallel alternating zones of temperature created by the conductive circuit,a sample addition pad on the first end of and in contact with the porous membrane,a fluid absorption pad on the opposing second end of and in contact with the porous membrane, anda heat sink placed on a side of the porous membrane opposing the conductive circuit.
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Abstract
This is a self-contained disposable thermal cycler device in which target sequence is amplified as the samples passes over a grid of alternating temperature and then at the completion of the reaction, the amplified material is captured by probes complementary to the targeted sequence. Since the device can be sealed and is disposable, it reduces the occurrence of cross contamination or specimen carry-over.
67 Citations
23 Claims
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1. A thermal strip thermocycler comprising:
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a substrate, a rectangular porous membrane, overlaying the substrate and enclosed by a clear impermeable non-electrically conductive material and having a first end and a second end opposing the first end, a conductive circuit printed on a surface of the substrate, the conductive circuit having parallel alternating zones of temperature produced by resistive heating elements positioned along the conductive circuit, and positioned such that a long axis and direction of flow within the porous membrane is perpendicular to the parallel alternating zones of temperature created by the conductive circuit, a sample addition pad on the first end of and in contact with the porous membrane, a fluid absorption pad on the opposing second end of and in contact with the porous membrane, and a heat sink placed on a side of the porous membrane opposing the conductive circuit. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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18. A thermal strip thermocycler comprising:
- a substrate,
a rectangular porous membrane, overlaying the substrate and enclosed by a clear impermeable non-electrically conductive material and having a first end and a second end opposing the first end, a conductive circuit printed on a surface of the substrate, the conductive circuit having parallel alternating zones of temperature produced by resistive heating elements positioned along the conductive circuit, and positioned such that a long axis and direction of flow within the porous membrane is perpendicular to the parallel alternating zones of temperature created by the conductive circuit, wherein adjacent segments of the conductive circuit have varying width dimensions, and wherein an initial segment of the conductive circuit is narrower than adjacent segments of a sinuous pattern thereof to provide a higher temperature for an initial cycle of thermocycling, a sample addition pad on the first end of and in contact with the porous membrane, and a fluid absorption pad on the second opposing end of and in contact with the porous membrane.
- a substrate,
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19. A thermal strip thermocycler comprising:
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a substrate, a rectangular porous membrane, overlaying the substrate and enclosed by a clear impermeable non-electrically conductive material and having a first end and a second end opposing the first end, a conductive circuit printed on a surface of the substrate, the conductive circuit having parallel alternating zones of temperature produced by resistive heating elements positioned along the conductive circuit, and positioned such that a long axis and direction of flow within the porous membrane is perpendicular to the parallel alternating zones of temperature created by the conductive circuit, wherein the conductive circuit includes at least two resistive circuits powered by different voltage sources and printed in an interlacing sinuous pattern allowing for additional temperature control, a sample addition pad on the first end of and in contact with the porous membrane, and a fluid absorption pad on the opposing second end of and in contact with the porous membrane. - View Dependent Claims (20)
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21. A thermal strip thermocycler comprising:
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a substrate comprising at least two low resistance conductive circuits printed in an intermeshing comb pattern on a surface thereof, a rectangular porous membrane, overlaying the substrate and enclosed by a clear impermeable non-electrically conductive material and having a first end and a second end opposing the first end, the conductive circuits having parallel alternating zones of temperature produced by resistive heating elements positioned along the conductive circuits and positioned such that a long axis and direction of flow within the porous membrane is perpendicular to the parallel alternating zones of temperature created by the conductive circuit, a sample addition pad on the first end of and in contact with the porous membrane, and a fluid absorption pad on the opposing second end of and in contact with the porous membrane.
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22. A thermal strip thermocycler comprising:
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a substrate comprising at least two low resistance circuits printed in an intermeshing comb pattern on a surface thereof with a closer spacing for a first tine of the intermeshing comb pattern of the at least two low resistance circuits to provide less electrical resistance and a higher temperature for an initial cycle of thermocycling, a rectangular porous membrane, overlaying the substrate and enclosed by a clear impermeable non-electrically conductive material and having a first end and a second end opposing the first end, the conductive circuits having parallel alternating zones of temperature produced by resistive heating elements positioned along the conductive circuits and positioned such that a long axis and direction of flow within the porous membrane is perpendicular to the parallel alternating zones of temperature created by the conductive circuit, a sample addition pad on the first end of and in contact with the porous membrane, and a fluid absorption pad on the opposing second end of and in contact with the porous membrane.
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23. A thermal strip thermocycler comprising:
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a substrate, a rectangular porous membrane, overlaying the substrate and enclosed by a clear impermeable non-electrically conductive material and having a first end and a second end opposing the first end, a conductive circuit printed on a surface of the substrate, the conductive circuit having parallel alternating zones of temperature produced by resistive heating elements positioned along the conductive circuit, and positioned such that a long axis and direction of flow within the porous membrane is perpendicular to the parallel alternating zones of temperature created by the conductive circuit, a sample addition pad on the first end of and in contact with the porous membrane, wherein a closeable gap separates the sample addition pad from the rectangular porous membrane such that sample can be incubated at a given time before flow of sample and reagents onto the porous membrane is initiated and flow can be initiated by sliding the sample addition pad and membrane either manually or by action of an instrument, and a fluid absorption pad on the opposing second end of and in contact with the porous membrane.
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Specification
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Current AssigneeJerome Anton Streifel, Raveendran Pottathil
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Original AssigneeJerome Anton Streifel, Raveendran Pottathil
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InventorsPottathil, Raveendran, Streifel, Jerome Anton
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Primary Examiner(s)Beisner; William H.
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Assistant Examiner(s)BOWERS, NATHAN ANDREW
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Application NumberUS10/382,755Publication NumberTime in Patent Office1,448 DaysField of SearchNoneUS Class Current435/287.2CPC Class CodesB01L 2300/0825 Test stripsB01L 2300/1827 using resistive heaterB01L 3/5023 with a sample being transpo...B01L 7/525 with physical movement of s...
