Ii-Key/antigenic epitope hybrid peptide vaccines
First Claim
1. An isolated nucleic acid molecule comprising:
- a) a first expressible nucleic acid sequence encoding a protein of interest or polypeptide of interest which contains a first MHC Class II-presented epitope; and
b) a second expressible nucleic acid sequence encoding an antigen presentation enhancing hybrid polypeptide comprising;
i) an N-terminal element consisting essentially of the residues LRMK (amino acids 1–
4) of SEQ ID NO.;
1 and 0–
12 additional sequential residues of SEQ ID NO.;
1, and modifications of SEQ ID No;
1 that retain antigen presentation enhancing activity;
ii) a C-terminal element comprising a second an MHC Class II-presented epitope, said second MHC Class II-presented epitope being in the form of a polypeptide or peptidomimetic structure which binds to the antigenic peptide binding site of an MHC class II molecule, the second MHC Class II-presented epitope being from the same protein or polypeptide of interest encoded by the nucleic acid sequence a); and
iii) an intervening peptidyl structure linking the N-terminal and C-terminal elements of the hybrid, the peptidyl structure having a length of about 20 amino acids or less.
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Accused Products
Abstract
Disclosed is a nucleic acid molecule comprising a first expressible sequence encoding a protein of interest or polypeptide of interest which contains an MHC Class II-presented epitope. In addition, the nucleic acid molecule comprises a second expressible nucleic acid sequence encoding an antigen presentation enhancing hybrid polypeptide. The antigen presentation enhancing hybrid polypeptide includes the following elements: i) an N-terminal element consisting essentially of 4–16 residues of the mammalian Ii-Key peptide LRMKLPKPPKPVSKMR (SEQ ID NO: 1) and non-N-terminal deletion modifications thereof that retain antigen presentation enhancing activity; ii) a C-terminal element comprising an MHC Class II-presented epitope in the form of a polypeptide or peptidomimetic structure which binds to the antigenic peptide binding site of an MHC class II molecule, the MHC Class II-presented epitope being contained in the protein of interest of step a); and iii) an intervening peptidyl structure linking the N-terminal and C-terminal elements of the hybrid, the peptidyl structure having a length of about 20 amino acids or less.
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Citations
8 Claims
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1. An isolated nucleic acid molecule comprising:
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a) a first expressible nucleic acid sequence encoding a protein of interest or polypeptide of interest which contains a first MHC Class II-presented epitope; and b) a second expressible nucleic acid sequence encoding an antigen presentation enhancing hybrid polypeptide comprising; i) an N-terminal element consisting essentially of the residues LRMK (amino acids 1–
4) of SEQ ID NO.;
1 and 0–
12 additional sequential residues of SEQ ID NO.;
1, and modifications of SEQ ID No;
1 that retain antigen presentation enhancing activity;ii) a C-terminal element comprising a second an MHC Class II-presented epitope, said second MHC Class II-presented epitope being in the form of a polypeptide or peptidomimetic structure which binds to the antigenic peptide binding site of an MHC class II molecule, the second MHC Class II-presented epitope being from the same protein or polypeptide of interest encoded by the nucleic acid sequence a); and iii) an intervening peptidyl structure linking the N-terminal and C-terminal elements of the hybrid, the peptidyl structure having a length of about 20 amino acids or less. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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Specification