Combinatorial selection of oligonucleotide aptamers
First Claim
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1. A NF-IL6 protein binding aptamer comprising a mix of unmodified and thiophosphate-modified nucleotides that form a partially thiophosphate-modified oligonucleotide identified by SEQ ID NO:
- 7, wherein at least one but no more than four of any one single nucleotide base is thiophosphate-modified, wherein the aptamer binds NF-IL6.
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Abstract
A random combinatorial selection method is disclosed for the construction of oligonucleotide aptamers in which nuclease resistance is conferred by the inclusion of modified nucleotides. The modified nucleotides are incorporated during PCR amplification to form achiral modified oligonucleotides. Thio-substituted aptamers are provided that bind tightly to the nuclear factor for human IL6 (NF-IL6).
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8 Claims
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1. A NF-IL6 protein binding aptamer comprising a mix of unmodified and thiophosphate-modified nucleotides that form a partially thiophosphate-modified oligonucleotide identified by SEQ ID NO:
- 7, wherein at least one but no more than four of any one single nucleotide base is thiophosphate-modified, wherein the aptamer binds NF-IL6.
- View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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