Gradient resolved information platform
First Claim
1. A hybridization platform comprising:
- a planar support; and
an immobilized layer of single stranded nucleic acids or nucleic acid analogs formed on the surface of the planar support;
wherein the immobilized layer is formed as either a) a continuous layer on the surface or b) as discrete regions on the surface;
the nucleic acids or nucleic acid analogs being arranged as the continuous layer, or as each region, such that the one or more parameters selected from the density, sequence, orientation or structure of the nucleic acid or nucleic acid analogs are spatially varied as a continuum in the continuous layer or in each region in one or both of a first and second dimension across the surface of the planar support so as to form at least one gradient of the one or more parameters within the continuous layer or each region; and
the one or more selected parameters includes the density of the nucleic acid or nucleic acid analogs.
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Accused Products
Abstract
Methods and devices for the detection and identification in a sample of one or more target molecules which bind to nucleic acid probe molecules are provided. The method includes contacting the sample with a surface that is coated with one or more gradients of nucleic acid or nucleic acid analog probe molecules that bind target molecules in the sample. Gradients are formed by varying a physical, structural or functional property of the probes on the surface; for example, the density of probe molecules bound to the surface. The coating layer or immobilization layer in which the gradient is formed is preferably continuous Determination of the location, speed and/or extent of hybridisation of a nucleic acid on a gradient surface is useful to identify target molecules bound to probes and/or to quantitatively measure the amount of the target in a sample.
23 Citations
30 Claims
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1. A hybridization platform comprising:
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a planar support; and an immobilized layer of single stranded nucleic acids or nucleic acid analogs formed on the surface of the planar support; wherein the immobilized layer is formed as either a) a continuous layer on the surface or b) as discrete regions on the surface; the nucleic acids or nucleic acid analogs being arranged as the continuous layer, or as each region, such that the one or more parameters selected from the density, sequence, orientation or structure of the nucleic acid or nucleic acid analogs are spatially varied as a continuum in the continuous layer or in each region in one or both of a first and second dimension across the surface of the planar support so as to form at least one gradient of the one or more parameters within the continuous layer or each region; and the one or more selected parameters includes the density of the nucleic acid or nucleic acid analogs. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25)
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26. A biosensor for detection of one or more target molecules comprising:
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a planar support; and an immobilized layer of single stranded nucleic acids or nucleic acid analogs formed on the surface of the planar support and capable of hybridizing to the one or more target molecules; wherein the immobilized layer is formed as either a) a continuous layer on the surface or b) as discrete regions on the surface; the nucleic acids or nucleic acid analogs being arranged as the continuous layer, or as each region, such that the one or more parameters selected from the density, sequence, orientation or structure of the nucleic acid or nucleic acid analogs are spatially varied as a continuum in the continuous layer or in each region in one or both of a first and second dimension across the surface of the planar support so as to form at least one gradient of the one or more parameters within the continuous layer or each region; and the one or more selected parameters includes the density of the nucleic acid or nucleic acid analogs. - View Dependent Claims (27)
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28. A kit for conducting an assay for detecting and quantifying one or more target molecules in a sample which comprises:
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a planar support; and an immobilized layer of single stranded nucleic acids or nucleic acid analogs formed on the surface of the planar support and capable of hybridizing to the one or more target molecules; wherein the immobilized layer is formed as either a) a continuous layer on the surface or b) as discrete regions on the surface; the nucleic acids or nucleic acid analogs being arranged as the continuous layer, or as each region, such that the one or more parameters selected from the density, sequence, orientation or structure of the nucleic acid or nucleic acid analogs are spatially varied as a continuum in the continuous layer or in each region in one or both of a first and second dimension across the surface of the planar support so as to form at least one gradient of the one or more parameters within the continuous layer or each region; and the one or more selected parameters includes the density of the nucleic acid or nucleic acid analogs. - View Dependent Claims (29)
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30. A method of forming a hybridization platform comprising:
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providing a planar support, and forming on the surface of the planar support an immobilized layer of single stranded nucleic acids or nucleic acid analogs, the immobilized layer being formed either as a) a continuous layer on the surface or b) as discrete regions on the surface, the continuous layer or each region being arranged such that one or more parameters selected from the density, sequence, orientation or structure of the nucleic acid or nucleic acid analogs are spatially varied as a continuum in the continuous layer or in each region in one or both of a first and second dimension across the surface of the planar support so as to form at least one gradient of the one or more parameters within the continuous layer or each region, wherein the one or more selected parameters includes the density of the nucleic acids or nucleic acid analogs.
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Specification