Pipetting module

• US 7,185,551 B2
• Filed: 10/24/2003
• Issued: 03/06/2007
• Est. Priority Date: 05/22/2003
• Status: Expired due to Fees

• Current Assignee: Jennifer Ackerman
• Original Assignee: H. Donald Schwartz
• Inventors: Schwartz, H. Donald
• Primary Examiner(s): RAEVIS, ROBERT R
• Application Number:
  US10/692,654
• Publication Number:
  US 20040231438A1
• Time in Patent Office: 1,229 Days
• Field of Search: 738/641.3, 738/641.6, 738/641.7, 738/641.8, 738/642.2, 738/642.3, 738/642.4, 738/642.5, 422/100, 436/180, 417/486, 417/487, 417/488
• US Class Current: 73/864.16
• CPC Class Codes: B01L 2200/023 adapted for different sizes of tubes, tips or container
• CPC Class Codes: B01L 2200/0615 Loss of fluid by dripping
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 3/0217 of the plunger pump type medical syringes A61M
• CPC Class Codes: B01L 3/022 Capillary pipettes, i.e. having very small bore B01L3/0224 - B01L3/0237 take precedence
• CPC Class Codes: B01L 3/0224 having mechanical means to set stroke length, e.g. movable stops B01L3/0231, B01L3/0234 take precedence
• CPC Class Codes: B01L 3/0231 having several coaxial pistons
• CPC Class Codes: G01F 11/023 with provision for varying the stroke of the piston
• CPC Class Codes: G01F 11/027 of the syringe type
• CPC Class Codes: G01N 2035/102 Preventing or detecting loss of fluid by dripping
• CPC Class Codes: G01N 2035/1039 Micropipettes, e.g. microcapillary tubes
• CPC Class Codes: G01N 35/1074 arranged in a two-dimensional array
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

An air-filled pipette for accurately metering small and large volumes of fluid samples is provided. The pipette has dual resolution capability such that the pipette can accurately aspirate a wide range of sample volumes and deliver them contact-free (touchless). The pipette may include an extension mandrel that reduces air space in a disposable tip within the pipette. The dual resolution capability and/or the extension mandrel also minimize errors associated with the compressibility of air. Multiple pipettes may also be arranged to form a pipetting module for the metering of multiple sample volumes simultaneously and automatically. The pipette includes a channel block having at least one cylindrical passage, that slides up and down over a rod, and a cylinder, with an axially extending passage therethrough, that is sized, shaped and aligned to fit into the cylindrical passage in a dynamic sealing relationship. A method for mixing multiple fluid samples within a pipette tip is also provided.

Claims

1. A pipette module comprising:

a channel block having at least one passage extending therethrough;

a rod sized, shaped and aligned to pass into the channel block passage;

a cylinder having a passage extending therethrough, the cylinder sized, shaped and aligned to pass into the channel block passage from the end opposite the end into which the rod passes;

a chamber defined by the channel block, rod, and cylinder, having a volume which is variable during the pipetting operation of the pipette module, with a first part of the chamber variable by relative movement of the channel block relative to the fixed relative positions of the rod and cylinder, and with a second part of the chamber variable by relative movement of the cylinder relative to the fixed relative positions of the rod and channel block;

a tip having a passageway therethrough, extending downwardly from the bottom of the cylinder; and

an extension mandrel with a passageway extending therethrough, the mandrel attached at an end of the cylinder such that the passageway extending through the mandrel is aligned with the passage extending through the cylinder, wherein the mandrel reduces the internal volume within the tip.

2. The pipette module of claim 1, wherein the extension mandrel is removably secured to the cylinder.

3. A pipette module comprising:

a channel block having at least one passage extending therethrough;

a rod sized, shaped and aligned to pass into the channel block passage;

a cylinder having a passage extending therethrough, the cylinder sized, shaped and aligned to pass into the channel block passage from the end opposite the end into which the rod passes;

a chamber defined by the channel block, rod, and cylinder, having a volume which is variable during the pipetting operation of the pipette module, with a first part of the chamber variable by relative movement of the channel block relative to the fixed relative positions of the rod and cylinder, and with a second part of the chamber variable by relative movement of the cylinder relative to the fixed relative positions of the rod and channel block; and

wherein the passage extending through the cylinder extends entirely through the cylinder from one end of the cylinder through to an opposite end of the cylinder.

4. The pipette module of claim 3, wherein a third part of the chamber is variable by relative movement of the rod relative to the fixed relative positions of the channel block and cylinder.

5. The pipette module of claim 4, wherein the diameter of the rod is less than the inner diameter of the channel block, providing an annular space between the inside surface of the channel block and the outside surface of the rod, defining said third part of the chamber.

6. The pipette module of claim 3, wherein the diameter of the rod is greater than the diameter of the cylinder, providing an annular space between the inner surface of the channel block and the outer surface of the cylinder, defining said first part of the chamber.

7. A pipette module comprising:

a channel block having at least one passage extending therethrough;

a rod sized, shaped and aligned to pass into the channel block passage;

a cylinder having a passage extending therethrough, the cylinder sized, shaped and aligned to pass into the channel block passage from the end opposite the end into which the rod passes;

a chamber defined by the channel block, rod, and cylinder, having a volume which is variable during the pipetting operation of the pipette module, with a first part of the chamber variable by relative movement of the channel block relative to the fixed relative positions of the rod and cylinder, and with a second part of the chamber is variable by relative movement of the cylinder relative to the simultaneously fixed relative positions of the rod and channel block.

8. The pipette module of claim 7, wherein a third part of the chamber is variable by relative movement of the rod relative to the fixed relative positions of the channel block and cylinder.

9. The pipette module of claim 8, wherein the diameter of the rod is less than the inner diameter of the channel block, providing an annular space between the inside surface of the channel block and the outside surface of the rod, defining said third part of the chamber.

10. The pipette module of claim 7, wherein the diameter of the rod is greater than the diameter of the cylinder, providing an annular space between the inner surface of the channel block and the outer surface of the cylinder, defining said first part of the chamber.

11. The pipette module of claim 7, wherein no portion of the rod extends into the passage extending through the cylinder.

12. The pipette module of claim 7, wherein at least a portion of the passage is axially extending through the cylinder.