3-hydroxypropionic acid and other organic compounds

• US 7,186,541 B2
• Filed: 11/20/2001
• Issued: 03/06/2007
• Est. Priority Date: 11/20/2000
• Status: Expired due to Fees

• Current Assignee: Cargill, Inc. (Agricultural Services Division)
• Original Assignee: Cargill, Inc. (Agricultural Services Division)
• Inventors: Gokarn, Ravi R., Gort, Steven John, Selifonova, Olga V., Selmer, Thorsten, Jessen, Holly Jean, Buckel, Wolfgang
• Primary Examiner(s): Saidha; Tekchand
• Application Number:
  US10/432,443
• Publication Number:
  US 20040076982A1
• Time in Patent Office: 1,932 Days
• Field of Search: 435/232, 536/23.2
• US Class Current: 435/232
• CPC Class Codes: C12N 15/52 Genes encoding for enzymes or proenzymes
• CPC Class Codes: C12N 9/0004 Oxidoreductases (1.)
• CPC Class Codes: C12P 7/18 polyhydric
• CPC Class Codes: C12P 7/40 containing a carboxyl group including Peroxycarboxylic acids
• CPC Class Codes: C12P 7/42 Hydroxy-carboxylic acids
• CPC Class Codes: C12P 7/52 Propionic acid; Butyric acids
• CPC Class Codes: C12P 7/625 Polyesters of hydroxy carboxylic acids
• CPC Class Codes: Y02P 20/52 using catalysts, e.g. selective catalysts

Abstract

Methods and materials related to producing 3-HP as well as other organic compounds are disclosed. Specifically, isolated nucleic acids, polypeptides, host cells, and methods and materials for producing 3-HP and other organic compounds are disclosed.

Claims

1. An isolated polypeptide comprising an amino acid sequence selected from the group consisting of:

(a) a sequence set forth in SEQ ID NO;

18;

(b) a portion of SEQ ID NO;

18 having 30 or more contiguous amino acid residues of SEQ ID NO;

18, wherein the portion of SEQ ID NO;

18 can function as an E2 alpha polypeptide subunit of an enzyme having lactyl-CoA dehydratase activity;

(c) the sequence set forth in SEQ ID NO;

18, containing 5 conservative substitutions; and

(d) the sequence set forth in SEQ ID NO;

18, containing 10 conservative substitutions.

2. An isolated polypeptide comprising the amino acid sequence set forth in SEQ ID NO:

18.

3. An isolated polypeptide consisting of the amino acid sequence set forth in SEQ ID NO:

18.

4. An isolated polypeptide comprising the amino acid sequence set forth in SEQ ID NO:

18 containing 5 conservative substitutions.

5. An isolated polypeptide comprising the amino acid sequence set forth in SEQ ID NO:

18 containing 10 conservative substitutions.

6. An isolated polypeptide comprising at least 95% sequence identity to SEQ ID NO:

18, wherein the polypeptide can function as an E2 alpha polypeptide subunit of an enzyme having lactyl-CoA dehydratase activity.

7. The isolated polypeptide of claim 6, wherein the polypeptide comprising at least 97% sequence identity to SEQ ID NO:

18, wherein the polypeptide can function as an E2 alpha polypeptide subunit of an enzyme having lactyl-CoA dehydratase activity.

8. The isolated polypeptide of claim 6, wherein the polypeptide comprising at least 98% sequence identity to SEQ ID NO:

18, wherein the polypeptide can function as an E2 alpha polypeptide subunit of an enzyme having lactyl-CoA dehydratase activity.

9. The isolated polypeptide of claim 6, wherein the polypeptide comprising at least 99% sequence identity to SEQ ID NO:

18, wherein the polypeptide can function as an E2 alpha polypeptide subunit of an enzyme having lactyl-CoA dehydratase activity.

10. An isolated polypeptide comprising a portion of SEQ ID NO:

18 having 30 or more contiguous amino acid residues of-SEQ ID NO;

18, wherein the portion of SEQ ID NO;

18 can function as an E2 alpha polypeptide subunit of an enzyme having lactyl-CoA dehydratase activity.