Low thermal mass fluorometer

• US 7,186,989 B2
• Filed: 06/03/2005
• Issued: 03/06/2007
• Est. Priority Date: 06/03/2004
• Status: Expired due to Fees

• Current Assignee: University of South Florida (State University System of Florida)
• Original Assignee: University of South Florida (State University System of Florida)
• Inventors: Fries, David P., Farmer, Andrew, Flanery, Bill
• Primary Examiner(s): Luebke; Renee
• Assistant Examiner(s): ZETTL, MARY E
• Application Number:
  US10/908,994
• Publication Number:
  US 20050269522A1
• Time in Patent Office: 641 Days
• Field of Search: 356/417, 250/458.1
• US Class Current: 250/458.1
• CPC Class Codes: G01N 21/0332 with temperature control control of temperature G05D23/00; cryostats F17C3/08
• CPC Class Codes: G01N 21/645 Specially adapted constructive features of fluorimeters
• CPC Class Codes: G01N 2201/0221 Portable; cableless; compact; hand-held

Abstract

The present invention provides core technologies necessary for a portable, low cost, thermal-regulating LED-based handheld fluorometer. The regulated fluorometer is based on a low thermal mass infrared heater, and an orthogonal geometry LED based filter fluorometer. Power is supplied through an external power supply and data is collected in real-time through standard serial interfaces of personal computers or personal digital assistants. Thermal regulation is automatically maintained using temperature sensor feedback control. Optical excitation relies on LED light source(s) and optical detection is through an adjustable integrating photodetector. Such a handheld system can allow applications requiring temperature sensitive photometric measurements for real time analyte detection to be performed in the field.

Claims

1. A low thermal mass fluorometer, the fluorometer comprising:

a reaction chamber;

an infrared radiant heater positioned to supply radiant heat to the reaction chamber;

an infrared thermometer positioned to monitor the radiant heat supplied to the reaction chamber by the infrared radiant heater;

a temperature feedback controller coupled to the infrared radiant heater and the infrared thermometer, the temperature feedback controller coupled to the infrared radiant heater to adjust the supply of radiant heat as monitored by the infrared thermometer;

a light emitting diode positioned to supply fluorescence excitation to the reaction chamber; and

an integrating photodetector positioned to detect fluorescence emission from the reaction chamber, the photodetector positioned geometrically orthogonal to the light emitting diode.

2. The fluorometer of claim 1, wherein the reaction chamber is dimensioned to accommodate a standard polymerase chain reaction tube.

3. The fluorometer of claim 1, wherein the infrared radiant heater further comprises a bandpass filter to pass infrared heat into the reaction chamber within a bandwidth that does not interfere with the light supplied by the light emitting diode.

4. The fluorometer of claim 1, wherein the infrared radiant heater is a pulse-width modulated infrared radiant heater.

5. The fluorometer of claim 1, wherein the infrared radiant heater is a MEMS-based infrared heater.

6. The fluorometer of claim 1, wherein the infrared thermometer is a thermopile based digital thermometer.

7. The fluorometer of claim 1, wherein the light emitting diode is a 5 mm Ti, blue light emitting diode.

8. The fluorometer of claim 1, further comprising a bandpass filter positioned to optically isolate the light emitted from the light emitting diode.

9. The fluorometer of claim 8, wherein the bandpass filter is a 485 nm bandpass filter.

10. The fluorometer of claim 1, wherein the integrating photodetector is a blue-enhanced integrating photodiode.

11. The fluorometer of claim 1, further comprising a bandpass filter positioned to optically isolate the fluorescence emission received by the integrating photodiode.

12. The fluorometer of claim 11, wherein the bandpass filter is a 505 nm bandpass filter.

13. The fluorometer of claim 1, further comprising a control software module in circuit communication with the temperature feedback controller, the light emitting diode and the integrating photodetector.

14. The fluorometer of claim 1, further comprising an external power supply.

15. The fluorometer of claim 1, further comprising an integrated power supply.

16. The fluorometer of claim 1, further comprising a serial communication module in circuit communication with the integrating photodetector.

17. The fluorometer of claim 12, further comprising an output display in communication with the serial communication module, the output display to present the fluorescence emitted from the reaction chamber.