Electrowetting electrode device with electromagnetic field for actuation of magnetic-bead biochemical detection system

• US 7,189,359 B2
• Filed: 07/28/2004
• Issued: 03/13/2007
• Est. Priority Date: 07/29/2003
• Status: Expired due to Fees

• Current Assignee: National Applied Research Laboratories (Government of The Republic of China)
• Original Assignee: National Tsing Hua University, Precision Instrument Development Center National Science Council
• Inventors: Yeh, Jer-Liang, Chen, Chien-Jen, Hu, Yi-Chiuen, Yuan, Shih-Jun, Yang, Jing-Tang, Yu, Chih-Sheng
• Primary Examiner(s): SINES, BRIAN J
• Application Number:
  US10/900,649
• Publication Number:
  US 20050056569A1
• Time in Patent Office: 958 Days
• Field of Search: 422/50, 422/55, 422/68.1, 422/82.01, 422/82.02, 436/43, 436/149, 436/63, 295/92, 295/921
• US Class Current: 422/82.01
• CPC Class Codes: G01N 27/745 for detecting magnetic beads used in biochemical assays concerning the assays G01N33/54326; sensors therefor G01R33/1269; automatic analysers therefor G01N35/0098
• CPC Class Codes: G01N 33/5438 Electrodes
• CPC Class Codes: Y10T 436/11 Automated chemical analysis

Abstract

A detecting device for biochemical detections is provided. The detecting device includes a first substrate, a magnetic layer located on the first substrate, an isolation layer located on the magnetic layer, at least a first electrode located on the isolation layer, a first dielectric layer located on the first electrode, a first hydrophobic layer located on the first dielectric layer, a second substrate, at least a second electrode located on the second substrate and having a cathode and an anode, a second dielectric layer located on the second electrode'"'"' and a second hydrophobic layer located on the second dielectric layer. The first electrode is zigzag-shaped, and the cathode and the anode of the second electrode are comb-shaped and interlaced with each other.

Claims

1. A magnetic beads biochemical detecting device for detecting a target in a plurality of magnetic beads of a droplet, comprising:

a first substrate;

a magnetic layer located on said first substrate;

an isolation layer located on said magnetic layer;

at least a first electrode located on said isolation layer, wherein said first electrode is zigzag-shaped;

a first dielectric layer located on said first electrode;

a first hydrophobic layer located on said first dielectric layer;

a second substrate;

at least a second electrode located on said second substrate and having a cathode and an anode, wherein said cathode and said anode are comb-shaped and are interlaced with each other;

a second dielectric layer located on said second electrode; and

a second hydrophobic layer located on said second dielectric layer;

wherein said first substrate and said second substrate are respectively configured to make said first electrode and said second electrode face to each other and to form a gap therebetween;

said droplet is movable in said gap by a voltage difference produced between said first electrode and said second electrode;

a magnetic force for attracting said plurality of magnetic beads is induced by applying currents only on said first electrode; and

a positive-and-negative interlaced electric field is generated by applying currents on said cathode and said anode for initiating a certain reaction of said target to further generate a signal of current to be detected.

2. The detecting device according to claim 1, wherein said magnetic layer is magnetized by said magnetic force.

3. The detecting device according to claim 1, wherein a magnetic line of force of said magnetic force is closed by said magnetic layer so as to reduce a dissipation of said magnetic line of force and enhance said magnetic force.

4. The detecting device according to claim 1, wherein said reaction is an oxidation-reduction reaction.

5. The detecting device according to claim 1, wherein said first substrate is a silicon substrate.

6. The detecting device according to claim 1, wherein said first electrode is made of a metal.

7. The detecting device according to claim 1, wherein said magnetic layer is made of a permalloy.

8. The detecting device according to claim 1, wherein said second substrate is a glass substrate.

9. The detecting device according to claim 1, wherein said second electrode is made of a transparent and electrically conductive material.

10. The detecting device according to claim 1, wherein said signal of current is forwarded by said cathode and said anode for being detected.