Methods and apparatuses of separating cells using magnets and droplet type cell suspension
First Claim
1. An apparatus for separating cells using magnets and droplet type cell suspension, comprising:
- a droplet type cell suspension forming part for forming a droplet type cell suspension under a lower part of the droplet type cell suspension forming part with cell suspension sample containing cells to which magnetic beads are coupled and wherein a region in which the droplet type cell suspension is to be formed, has a droplet type cell suspension forming part having a hydrophilic property and another region of the droplet type cell suspension forming part has a hydrophobic property;
a cell suspension inlet for supplying the droplet type cell suspension forming part with the cell suspension sample;
a magnet for applying magnetic force to the droplet type cell suspension;
a cell buffer inlet for supplying the droplet type cell suspension with cell buffer; and
a temperature maintaining part for maintaining the temperature of the droplet type cell suspension.
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Abstract
Disclosed are methods and apparatuses for separating cells using magnets and droplet type cell suspension according to the present invention, which may effectively separate cells by forming droplet type cell suspension with cell suspension sample containing cells to which magnetic beads are coupled and applying magnetic force to the droplet type cell suspension.
The apparatus of separating cells according to the present invention includes: a droplet type cell suspension forming part for forming a droplet type cell suspension under a lower part of the droplet type cell suspension forming part with cell suspension sample containing cells to which magnetic beads are coupled; a cell suspension inlet for supplying the droplet type cell suspension forming part with the cell suspension sample; a magnet for applying magnetic force to the droplet type cell suspension; a cell buffer inlet for supplying the droplet type cell suspension with cell buffer; and a temperature maintaining part for maintaining a temperature of the droplet type cell suspension.
67 Citations
13 Claims
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1. An apparatus for separating cells using magnets and droplet type cell suspension, comprising:
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a droplet type cell suspension forming part for forming a droplet type cell suspension under a lower part of the droplet type cell suspension forming part with cell suspension sample containing cells to which magnetic beads are coupled and wherein a region in which the droplet type cell suspension is to be formed, has a droplet type cell suspension forming part having a hydrophilic property and another region of the droplet type cell suspension forming part has a hydrophobic property; a cell suspension inlet for supplying the droplet type cell suspension forming part with the cell suspension sample; a magnet for applying magnetic force to the droplet type cell suspension; a cell buffer inlet for supplying the droplet type cell suspension with cell buffer; and a temperature maintaining part for maintaining the temperature of the droplet type cell suspension. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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12. An apparatus for separating cells using magnets and droplet type cell suspension, comprising:
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a droplet type cell suspension forming part for forming a droplet type cell suspension under a lower part of the droplet type cell suspension forming part with cell suspension sample containing cells to which magnetic beads are coupled and wherein a region in which the droplet type cell suspension is to be formed, has a droplet type cell suspension forming part having a hydrophilic property and another region of the droplet type cell suspension forming part has a hydrophobic property; a cell suspension inlet for supplying the droplet type cell suspension forming part with the cell suspension sample; a magnet for applying magnetic force to the droplet type cell suspension; a cell buffer inlet for supplying the droplet type cell suspension with cell buffer and regulating vertical location of the cell buffer inlet itself; and a droplet type cell suspension protecting part, connected to the outlet and spaced apart from the droplet type cell suspension forming part by a predetermined distance, for protecting the droplet type cell suspension. - View Dependent Claims (13)
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Specification