Method for measuring substance and testing piece

• US 7,189,576 B2
• Filed: 03/11/2003
• Issued: 03/13/2007
• Est. Priority Date: 10/30/1995
• Status: Expired due to Fees

• Current Assignee: ARKRAY, Inc
• Original Assignee: ARKRAY, Inc
• Inventors: Yamamoto, Kenji, Yonehara, Satoshi, Fukuoka, Takao, Katayama, Atsuko
• Primary Examiner(s): Warden; Jill
• Assistant Examiner(s): SIEFKE, SAMUEL P
• Application Number:
  US10/384,757
• Publication Number:
  US 20030175985A1
• Time in Patent Office: 1,463 Days
• Field of Search: 422/56, 422/57, 436/164, 436/169, 436/178
• US Class Current: 436/170
• CPC Class Codes: C12Q 1/28 involving peroxidase
• CPC Class Codes: G01N 21/272 for following a reaction, e.g. for determining photometrically a reaction rate (photometric cinetic analysis)
• CPC Class Codes: G01N 21/78 producing a change of colour
• CPC Class Codes: G01N 31/00 Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods
• CPC Class Codes: G01N 31/22 using chemical indicators G01N31/02 takes precedence
• CPC Class Codes: G01N 33/525 Multi-layer analytical elements
• CPC Class Codes: G01N 33/54393 Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
• CPC Class Codes: Y10T 436/23 Carbon containing
• CPC Class Codes: Y10T 436/255 including use of a solid sorbent, semipermeable membrane, or liquid extraction

Abstract

A method of measuring an analyte, comprising a step of measuring a detectable substance by using a reaction system including a formation reaction of the detectable substance based on a chemical reaction of the analyte contained in a sample, wherein a layered inorganic compound is caused to exist in the reaction system including the formation reaction of the detectable substance, whereby high-sensitivity measurement is made possible, the detectable substance can be stabilized to improve accuracy of the measurement, a rate of a chemical reaction is increased to enable quick measurement, and high-sensitivity measurement is made possible even in a reaction system which forms an insoluble substance. Also, it can be provided an analytical testing piece for measuring an analyte, by measuring a detectable substance by using a reaction system including a formation reaction of the detectable substance based on a chemical reaction of the analyte contained in a sample, wherein the testing piece comprises at least one test portion having a detection portion for detecting the detectable substance and contains a layered inorganic compound at least in the test portion, whereby diffusion and elution of a dyestuff or the like is prevented, more sensitive and accurate simple, analysis is made possible, and easy handling is possible.

Claims

1. A method for measuring an analyte, comprising a step of measuring a detectable substance by using an aqueous reaction system including a formation reaction of the detectable substance based on a chemical reaction of the analyte contained in a sample, wherein a layered inorganic compound is dispersed in the aqueous reaction system to allow the layered inorganic compound to adsorb the detectable substance, thereby increasing measurement sensitivity of the detectable substance.

2. The method according to claim 1, wherein the layered inorganic compound is a 2:

1 type clay mineral.

3. The method according to claim 2, wherein the 2:

1 type clay mineral is a swelling layered clay mineral.

4. The method according to claim 3, wherein the swelling layered clay mineral is at least one member selected from the group consisting of bentonite, smectite, vermicullite and synthetic fluoro-mica.

5. The method according to claim 4, wherein the smectite is synthetic smectite.

6. The method according to claim 5, wherein the synthetic smectite is at least one member selected from the group consisting of hectorite and saponite.

7. The method according to claim 1, wherein the detectable substance can be detected by an optical method or an electrochemical method.

8. The method according to claim 7, wherein the detectable substance is a dyestuff detectable by an optical method.

9. The method according to claim 8, wherein the dyestuff is an azo compound.

10. The method according to claim 7, wherein the detectable substance is a compound detectable by an electrochemical method and selected from the group consisting of an electron carrier, an electron donor and an electron receptor.

11. The method according to claim 1, wherein the detectable substance is a compound selected from the group consisting of amines, imines, polyenes, aromatic compounds, heterocyclic compounds and complexes between ions and cyclic ligands.

12. The method according to claim 1, wherein the formation reaction of the detectable substance is an oxidation-reduction reaction.

13. The method according to claim 12, wherein the formation reaction of the detectable substance is an oxidation-reduction reaction and the reaction system including the formation reaction of the detectable substance includes a formation reaction of hydrogen peroxide or an oxidation reaction using hydrogen peroxide as an oxidizing agent.

14. The method according to claim 12, wherein the formation reaction of the detectable substance is an oxidation-reduction reaction and the reaction system including the formation reaction of the detectable substance includes the formation reaction of nicotinamide adenine dinucleotide or nicotinamide adenine dinucleotide phosphate or a reaction in which nicotinamide adenine dinucleotide or nicotinamide adenine dinucleotide phosphate acts as a reducing agent.

15. The method according to claim 1, wherein a buffer or a dried product thereof is contained in the reaction system.

16. The method according to claim 1, wherein the layered inorganic compound in the reaction system is added in the form of dispersion to the reaction system.