High performance fluorescent optical sensor

• US 7,190,445 B2
• Filed: 06/24/2005
• Issued: 03/13/2007
• Est. Priority Date: 12/11/2001
• Status: Expired due to Term

• Current Assignee: Senseonics, Incorporated (Senseonics Holdings, Inc.)
• Original Assignee: Sensors For Medicine and Science Incorporated
• Inventors: Colvin, Arthur E. Jr., Walters, Steven J.
• Primary Examiner(s): Lauchman; Layla G.
• Application Number:
  US11/165,129
• Publication Number:
  US 20050237518A1
• Time in Patent Office: 627 Days
• Field of Search: 356/218, 356/436, 356/244, 356/410, 356/417, 356/317, 356/318, 356/234, 385/12, 385129-131, 250/458.1, 250/459.1, 422/82.05, 422/82.08, 436/164, 436/527
• US Class Current: 356/218
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/645 Specially adapted constructive features of fluorimeters
• CPC Class Codes: G01N 21/7703 using reagent-clad optical fibres or optical waveguides using measurement of total internal reflection or attenuated total reflection G01N21/552; optical fibres or waveguides per se G02B
• CPC Class Codes: G01N 2201/0642 Light traps; baffles

Abstract

An optical sensor device for determining the presence or concentration of an analyte, contains a waveguide disposed over a light source and a light detector mounted on a surface of a substrate and separated by an internal baffle, wherein the waveguide has a thickness corresponding to a far field emission point of the light source as determined by a light shielding baffle between the light source and light detector. An analyte indicator matrix is disposed on the outer surface of the waveguide. The sensor device geometry takes advantage of only direct illumination of the indicator matrix, and direct collection of indicator matrix illumination, without any significant reflection by said waveguide. Undesirable light noise generated by the light source passes directly out of the device through the waveguide.

Claims

1. An optical sensor device for determining the presence or concentration of an analyte, comprising:

a substrate;

a light source for emitting light primarily of a preselected wavelength upon energization, mounted on a surface of said substrate;

a light detector for detecting light incident thereon and generating an electrical signal responsive thereto, mounted on said surface adjacent to said light source;

a waveguide formed over said light source and said light detector;

an analyte-permeable indicator matrix disposed on an outer surface of said waveguide, said indicator matrix containing light-emitting indicator molecules whose light emission is attenuated or enhanced by the presence of analyte in said matrix, said indicator molecule being selected such that the wavelength emitted by the light source excites light emission in the indicator molecules; and

a transparent fill-in material formed over said light source in a cavity in which said light source is located, wherein said transparent fill-in material has a refractive index that is substantially equal to the refractive index of said waveguide, such that said light source is in effect mounted within said waveguide;

wherein said light detector generates an electrical signal responsive to light incident thereon emitted by said indicator molecules.

2. An optical sensor device according to claim 1, wherein said fill-in material is a polymeric material.

3. An optical sensor device for determining the presence or concentration of an analyte, comprising:

a substrate;

a light source for emitting light primarily of a preselected wavelength upon energization, mounted on a surface of said substrate;

a light detector for detecting light incident thereon and generating an electrical signal responsive thereto, mounted on said surface adjacent to said light source, and being separated therefrom by a light shielding baffle, said baffle defining at least two cavities in which said light source and said light detector are located when mounted on said substrate;

a waveguide formed over said light source, said light detector and cavities;

an analyte-permeable indicator matrix disposed on an outer surface of said waveguide, said indicator matrix containing light-emitting indicator molecules whose light emission is attenuated or enhanced by the presence of analyte in said matrix, said indicator molecule being selected such that the wavelength emitted by the light source excites light emission in the indicator molecules; and

a color-doped fill-in material formed over said photodetector in the cavity in which said photodetector is located, wherein said color-doped fill-in material functions as a filter to block a predominant wavelength emitted by said light source from impinging on said photodetector;

wherein said light detector generates an electrical signal responsive to light incident thereon emitted by said indicator molecules.

4. An optical sensor device according to claim 3, wherein said color-doped fill-in material has a refractive index that is higher than the refractive index of said waveguide.