Imaging systems for fluorescence and reflectance imaging and spectroscopy and for contemporaneous measurements of electromagnetic radiation with multiple measuring devices

• US 7,190,452 B2
• Filed: 02/14/2005
• Issued: 03/13/2007
• Est. Priority Date: 12/19/2000
• Status: Expired due to Fees

• Current Assignee: Verisante Technology Inc.
• Original Assignee: Perceptronix Medical Inc.
• Inventors: Zeng, Haishan, Lam, Stephen, Palcic, Branko Mihael
• Primary Examiner(s): Toatley, Jr.; Gregory J.
• Assistant Examiner(s): GEISEL, KARA E
• Application Number:
  US11/058,045
• Publication Number:
  US 20050203423A1
• Time in Patent Office: 757 Days
• Field of Search: 356 72- 73, 356/326
• US Class Current: 356/326
• CPC Class Codes: G01J 3/02 Details
• CPC Class Codes: G01J 3/021 using plane or convex mirrors, parallel phase plates, or particular reflectors
• CPC Class Codes: G01J 3/0218 using optical fibers
• CPC Class Codes: G01J 3/0235 using means for replacing an element by another, for replacing a filter or a grating
• CPC Class Codes: G01J 3/0243 having a through-hole enabling the optical element to fulfil an additional optical function, e.g. a mirror or grating having a throughhole for a light collecting or light injecting optical fiber
• CPC Class Codes: G01J 3/0289 Field-of-view determination; Aiming or pointing of a spectrometer; Adjusting alignment; Encoding angular position; Size of measurement area; Position tracking
• CPC Class Codes: G01J 3/10 Arrangements of light sources specially adapted for spectrometry or colorimetry
• CPC Class Codes: G01J 3/36 Investigating two or more bands of a spectrum by separate detectors
• CPC Class Codes: G01N 2223/076 X-ray fluorescence
• CPC Class Codes: G01N 23/223 by irradiating the sample with X-rays or gamma-rays and by measuring X-ray fluorescence

Abstract

Optical systems that provide for simultaneous images and spectra from an object, such as a tissue sample, an industrial object such as a computer chip, or any other object that can be viewed with an optical system such as a microscope, endoscope, telescope or camera. In some embodiments, the systems provide multiple images corresponding to various desired wavelength ranges within an original image of the object, as well as, if desired, directional pointer(s) that can provide both an identification of the precise location from which a spectrum is being obtained, as well as enhancing the ability to point the device.

Claims

1. A system means for detecting a light beam comprising:

an area means for receiving the light beam;

a means for spatially separating the light beam disposed in the area means to separate a small spatial portion of the light beam from an adjacent remainder of the light beam to provide a separated light beam and a remainder light beam and means to transmit the separated light beam to a means for spectroscopy;

a means for imaging disposed in the area means to operably receive the remainder light beam to provide an image therefrom; and

,

a means for spectroscopy optically connected to the means for separating the light beam to receive the separated light beam to provide a spectrum therefrom, wherein the means for spectroscopy is located outside of the light beam and the means for spatially separating the light beam comprises a means for light redirection sized and located to intercept a small area of the light beam and change the direction of such small area toward the means for spectroscopy, and wherein the means for light redirection separates substantially all light incident thereon from the remainder light beam and imparts a small residual image in the remainder light beam corresponding to the location of the means for light redirection in the light beam.

3. The means for detecting a light beam of claim 1 or 2 wherein the means for detecting a light beam further comprises a means for displaying operably connected to the means for imaging to display an image from the means for imaging.

4. The means for detecting a light beam of claim 1 or 2 wherein the system further comprises a means for displaying operably connected to the means for spectroscopy to display a spectrum from the means for spectroscopy.

5. The means for detecting a light beam of claim 1 or 2 wherein the system further comprises a means for image separating comprising a plurality of means for selecting light that separates the image into a plurality of selected wavelength region images wherein each selected wavelength region image corresponds to a different wavelength region of the range of wavelengths in the light beam.

6. The means for detecting a light beam of claim 1 or 2 wherein the means for detecting comprises a means for controlling operably connected to an imaging detector and the means for spectroscopy and containing computer-implemented programming that controls the means for imaging and the means for spectroscopy.

2. A system means for detecting a light beam comprising:

an area means for receiving the light beam;

a means for spatially separating the light beam disposed in the area means to separate a small spatial portion of the light beam from a an adjacent remainder of the light beam to provide a separated light beam and a remainder light beam and means to transmit the separated light beam to a means for spectroscopy;

a means for imaging disposed in the area means to operably receive the remainder light beam to provide an image therefrom; and

,

a means for spectroscopy optically connected to the means for separating the light beam to receive the separated light beam to provide a spectrum therefrom, wherein the means for spatially separating the light beam is a means for splitting the light beam that transmits at least about 80% of the light beam and reflects at least about 20% of the light beam.