Real-time biofilm monitoring system

• US 7,190,457 B2
• Filed: 01/13/2004
• Issued: 03/13/2007
• Est. Priority Date: 01/13/2004
• Status: Active Grant

• Current Assignee: Echo Technologies Incorporated
• Original Assignee: Echo Technologies Incorporated
• Inventors: Tabacco, Mary Elizabeth, Schanzie, J. Anthony, Mittelman, Marc W
• Primary Examiner(s): Lee; Hwa (Andrew)
• Assistant Examiner(s): GEISEL, KARA E
• Application Number:
  US10/758,318
• Publication Number:
  US 20050151971A1
• Time in Patent Office: 1,155 Days
• Field of Search: 356/417, 250/461.2
• US Class Current: 356/417
• CPC Class Codes: G01N 17/008 Monitoring fouling
• CPC Class Codes: G01N 2021/6441 with two or more labels
• CPC Class Codes: G01N 2021/6482 Sample cells, cuvettes
• CPC Class Codes: G01N 2021/6484 Optical fibres
• CPC Class Codes: G01N 2021/8411 Application to online plant, process monitoring
• CPC Class Codes: G01N 2021/8528 Immerged light conductor
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/645 Specially adapted constructive features of fluorimeters
• CPC Class Codes: G01N 21/763 Bioluminescence
• CPC Class Codes: G01N 21/8422 Investigating thin films, e.g. matrix isolation method
• CPC Class Codes: G01N 21/8507 Probe photometers, i.e. with optical measuring part dipped into fluid sample
• CPC Class Codes: G01N 2201/0846 Fibre interface with sample, e.g. for spatial resolution
• CPC Class Codes: G01N 2201/1296 using neural networks

Abstract

Real time biofilm monitoring systems are provided. Said systems comprise single or multiple fiber-optic probes detecting wavelength-specific fluorescence from biomarkers of fouling organisms; a compact optoelectronic interface and data acquisition system interfaced with said probes, wherein said probe or probes are bifurcated and contain at least one excitation and at least one emission filter permitting the simultaneous resolution of multiple biomarkers.

Claims

1. A real-time biofilm monitoring system comprising:

at least one fiber-optic probe that detects fluorescence of an inherent biomarker of a fouling organism, and

a compact optoelectronic interface and data acquisition system interfaced with said probe, wherein said probe is bifurcated and contains at least one excitation and at least one emission filter capable of resolution of multiple emission wavelengths that can be correlated to the presence or absence of an inherent biomarker.

2. The real-time biofilm monitoring system of claim 1, comprising multiple fiber-optic probes.

3. The real-time biofilm monitoring system according to claim 1, further comprising an excitation reference channel to correct for spectral interference from non-biological materials.

4. The real-time bioflim monitoring system according to claim 2, further comprising an excitation reference channel to correct for spectral interference from non-biological materials.

5. The real-time biofilm monitoring system of claim 1, wherein the system detects fluorescence in situ, in-line, or external to a sample.

6. The real-time biofilm monitoring system of claim 1, wherein the system does not include additional reagents or consumables.

7. The real-time biofilm monitoring system of claim 1, wherein the biomarker is selected from the group consisting of an amino acid, ATP, NADH, chlorophyll, and bioluminescence.

8. The real-time biofilm monitoring system of claim 7, wherein the amino acid is selected from the group consisting of Trp, Tyr, and Phe.

9. The real-time biofilm monitoring system of claim 7, further comprising detecting % transmission of excitation light.

10. The real-time biofilm monitoring system of claim 8, wherein a change in the % transmission of excitation light can be correlated to scaling due to non-biological materials selected from the group consisting of CaCO₃ and MgSO₄.

11. The real-time biofilm monitoring system of claim 1, wherein the system is capable of simultaneous resolution of multiple inherent biomarkers.

12. The real-time biofilm monitoring system of claim 1, wherein the system detects fluorescence of at least one inherent biomarker using multiple excitation wavelengths or multiple emission wavelengths.

13. The real-time biofilm monitoring system of claim 1, wherein the probe performs direct fluorescence detection of a inherent biomarker.

14. The real-time biofilm monitoring system of claim 1, wherein the system measures sloughing of a biofilm from a substrate.

15. A method for detecting a fouling organism comprising detecting fluorescence of an inherent biomarker comprising:

a. introducing excitation light into a first side of a bifurcated optical fiber bundle directed at a sample;

b. obtaining an emission arising from a substrate through a second side of a bifurcated optical fiber bundle; and

c. detecting the emission from the substrate and correlating the emission to the presence or absence of a fouling organism.

16. The method according to claim 15, wherein the fouling organism is P. Aeruginosa.

17. The method according to claim 15, wherein the sample is selected from the group consisting of a process fluid, a heat exchange system, a utility plant, a microelectronics fabrication system, a food processing system, a fluid handling system, and pulp and paper processing system.

18. The method according to claim 15, wherein the sample comprises a substrate selected from the group consisting of glass, polycarbonate, metal, and paint.

19. The method according to claim 16, wherein the sample comprises a substrate selected from the group consisting of glass, polycarbonate, metal, and paint.

20. The method of claim 15, wherein the emission from a bulk phase bacteria is distinguishable from emission from the biofilm.

21. The method of claim 15, wherein the method is performed in situ, in-line, or external to a sample.

22. The method of claim 15, wherein the biomarker is selected from the group consisting of an amino acid, ATP, NADH chlorophyll, and bioluminescence.

23. The method of claim 22, wherein the amino acid is selected from the group consisting of Trp, Tyr, and Phe.

24. The method of claim 15, further comprising detecting % transmission of excitation light.

25. The method of claim 15, wherein the system is capable of simultaneous resolution of multiple inherent biomarkers.

26. The method of claim 15, wherein multiple excitation wavelengths are introduced or multiple emission wavelengths are detected.

27. The method of claim 15, wherein the method comprises direct fluorescence detection of a inherent biomarker.

28. The method of claim 15, further comprising distinguishing a bulk phase bacteria from the biofilm.

29. A method for detecting a plurality of fouling organisms comprising detecting fluorescence of an inherent biomarker comprising:

a. introducing excitation light into a plurality of first sides of a plurality of bifurcated optical fiber bundles directed at a sample, wherein the excitation light can be the same or different for each first side of the bifurcated optical fiber bundles;

b. obtaining emissions arising from a substrate through second sides of a plurality of bifurcated optical fiber bundles; and

c. detecting the emission from the substrate and correlating this emission to the presence or absence of fouling organisms.

30. The method according to claim 29, wherein the sample is selected from the group consisting of a process fluid, a heat exchange system, a utility plant, a microelectronics fabrication system, a food processing system, a fluid handling system, and pulp and paper processing system.