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Methods and systems for releasing intracellular material from cells within microfluidic samples of fluids

  • US 7,192,557 B2
  • Filed: 12/14/2001
  • Issued: 03/20/2007
  • Est. Priority Date: 03/28/2001
  • Status: Expired due to Term
First Claim
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1. A microfluidic device comprising:

  • a lysing module configured to receive a cell-containing microdroplet;

    an actuator located upstream of the lysing module and configured to create a difference between an upstream pressure and a downstream pressure acting on the cell-containing microdroplet to move the microdroplet towards the lysing module;

    a vented positioning element located upstream of the lysing module and downstream of the actuator, wherein the vented positioning element is configured to stop the cell-containing microdroplet in a lysing position with respect to the lysing module, and wherein the vented positioning element is configured to position a portion of the cell-containing microdroplet downstream of the vented positioning element in the lysing position; and

    a lysing mechanism within the lysing module, configured to release intracellular material from cells within the cell-containing microdroplet in the lysing position with respect to the lysing module.

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