Multi-well rotary synthesizer

• US 7,192,558 B2
• Filed: 12/19/2000
• Issued: 03/20/2007
• Est. Priority Date: 06/16/1998
• Status: Expired due to Term

• Current Assignee: Mcluen Design Incorporated
• Original Assignee: Mcluen Design Incorporated
• Inventors: McLuen, Gary R., Hugens, Daniel W., Hanney, Richard J.
• Primary Examiner(s): SINES, BRIAN J
• Application Number:
  US09/742,261
• Publication Number:
  US 20010000723A1
• Time in Patent Office: 2,282 Days
• Field of Search: 422129-131, 422 99-104, 422 62- 67, 137/256, 137/602, 436176-178, 436/180, 436/43, 436/45, 436/47, 436/48, 436/49, 436/63, 436/179
• US Class Current: 422/510
• CPC Class Codes: B01J 19/00 Chemical, physical or physico-chemical processes in general; Their relevant apparatus
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00286 Reactor vessels with top and bottom openings
• CPC Class Codes: B01J 2219/00308 interchangeably mounted in racks or blocks
• CPC Class Codes: B01J 2219/00326 Movement by rotation
• CPC Class Codes: B01J 2219/00351 Means for dispensing and evacuation of reagents
• CPC Class Codes: B01J 2219/00353 Pumps
• CPC Class Codes: B01J 2219/00423 using filtration, e.g. through porous frits
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/0059 Sequential processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00689 using computers
• CPC Class Codes: B01J 2219/0072 Organic compounds
• CPC Class Codes: C40B 60/14 for creating libraries
• CPC Class Codes: G01N 35/025 having a carousel or turntable for reaction cells or cuvettes
• CPC Class Codes: Y10T 436/11 Automated chemical analysis
• CPC Class Codes: Y10T 436/111666 Utilizing a centrifuge or compartmented rotor
• CPC Class Codes: Y10T 436/113332 with conveyance of sample along a test line in a container or rack
• CPC Class Codes: Y10T 436/114165 with step of insertion or removal from test line
• CPC Class Codes: Y10T 436/114998 with treatment or replacement of aspirator element [e.g., cleaning, etc.]
• CPC Class Codes: Y10T 436/25375 : Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
• CPC Class Codes: Y10T 436/255 : including use of a solid sorbent, semipermeable membrane, or liquid extraction
• CPC Class Codes: Y10T 436/25625 : Dilution
• CPC Class Codes: Y10T 436/2575 : Volumetric liquid transfer

Abstract

An apparatus for synthesizing polymer chains includes a controller, a plurality of precision fit vials circularly arranged in multiple banks on a cartridge, a drain corresponding to each bank of vials, a chamber bowl, a plurality of valves for delivering reagents to selective vials, and a waste tube system for purging material from the vials. A purging operation can be selectively performed on one or more of the banks of vials. The multiple banks of valves provide an additional number of reagent choices while operating in a serial mode and faster reagent distribution while operating in a parallel mode. The plurality of vials are stored in the cartridge and are divided among individual banks wherein each bank of vials has a corresponding drain. There is at least one waste tube system for expelling the reagent solution from vials within a particular bank of vials when the waste tube system is coupled to the corresponding drain. The cartridge holding the plurality of vials rotates relative to the stationary banks of valves and the waste tube system. The controller rotates the cartridge and operates the banks of valves and the waste tube system in response to the required sequence of dispensing various reagent solutions and flushing appropriate vials in order to form the desired polymer chain within each vial.

Claims

1. A purging system within a synthesizer, the synthesizer further comprising a first bank of vials and a second bank of vials wherein the first bank of vials includes a first plurality of vials and has a first drain and the second bank of vials includes a second plurality of vials and has a second drain, the purging system comprising:

a. a pressurizing system for creating a pressure differential within a selective one of the first bank of vials and the second bank of vials; and

b. a first waste tube movable to couple to a selective one of the first drain to purge material from all of the first plurality of vials within the first bank of vials and the second drain to purge material from all of the second plurality of vials within the second bank of vials.

2. The purging system according to claim 1 further comprising a drain seal coupled to the first waste tube for creating a flexible seal between the first waste tube and the selective one of the first drain and the second drain.

3. The purging system according to claim 1 further comprising a second waste tube capable of selectively engaging the first drain to purge the material from all of the first plurality of vials within the first bank of vials and the second drain to purge the material from all of the second plurality of vials within the second bank of vials wherein the purging system is capable of selectively and simultaneously purging the first bank of vials and the second bank of vials.

8. The purging system according to claim 1 wherein the means for purging further includes a second waste tube capable of selectively engaging the first drain to purge the material from all of the first plurality of vials within the first bank of vials and the second drain to purge the material from all of the second plurality of vials within the second bank of vials wherein the purging system is capable of selectively and simultaneously purging the first bank of vials and the second bank of vials.

4. A purging system within a synthesizer, the synthesizer further comprising a first bank of vials and a second bank of vials wherein the first bank of vials includes a first plurality of vials and has a first drain and the second bank of vials includes a second plurality of vials and has a second drain, the purging system comprising:

a. means for generating a pressure differential within a selective one of the first bank of vials and the second bank of vials; and

b. means for purging for coupling to a selective one of the first drain for purging material from all of the first plurality of vials within the first bank of vials and the second drain to purge material from all of the second plurality of vials within the second bank of vials, wherein the means for purging is movable to the first drain and to the second drain.

5. The purging system according to claim 4 further comprising means for sealing coupled to the means for purging for generating a flexible seal between the means for purging and the selective one of the first drain and the second drain.

6. The purging system according to claim 5 wherein the means for sealing includes a drain seal.

7. The purging system according to claim 4 wherein the means for purging includes a first waste tube.

9. The purging system according to claim 4 wherein the means for generating includes a pressurizing system.

10. A purging system within a synthesizer, the synthesizer further comprising a first bank of vials including a first plurality of vials and having a first drain and a second bank of vials including a second plurality of vials and having a second drain, the purging system comprising:

a. a pressurizing system to generate a pressure differential within a selective one of the first bank of vials and the second bank of vials;

b. a first waste tube movable to couple to a selective one of the first drain to purge material from all of the first plurality of vials within the first bank of vials and the second drain to purge material from all of the second plurality of vials within the second bank of vials; and

c. a drain seal coupled to the first waste tube for generating a flexible seal between the first waste tube and the selective one of the first drain and the second drain.

11. The purging system according to claim 10 further comprising a second waste tube capable of selectively engaging the first drain to purge the material from all of the first plurality of vials within the first bank of vials and the second drain to purge the material from all of the second plurality of vials within the second bank of vials wherein the purging system is capable of selectively and simultaneously purging the first bank of vials and the second bank of vials.