System for optically sectioning and mapping surgically excised tissue

• US 7,194,118 B1
• Filed: 11/10/2000
• Issued: 03/20/2007
• Est. Priority Date: 11/10/2000
• Status: Expired due to Term

• Current Assignee: Caliber Imaging & Diagnostics, Inc.
• Original Assignee: Lucid Inc.
• Inventors: Zavislan, James M., Harris, Duncan
• Primary Examiner(s): AHMED, SAMIR ANWAR
• Application Number:
  US10/129,719
• Time in Patent Office: 2,321 Days
• Field of Search: 382/128, 382/133, 382/134, 382/110, 382/284, 382/297, 359/384, 359/385, 359/388, 359/393, 359/398, 600/300, 600/407, 600/476, 600/478
• US Class Current: 382/128
• CPC Class Codes: G06T 2200/32 involving image mosaicing
• CPC Class Codes: G06T 2207/10056 Microscopic image
• CPC Class Codes: G06T 2207/20016 Hierarchical, coarse-to-fine, multiscale or multiresolution image processing; Pyramid transform
• CPC Class Codes: G06T 2207/20092 Interactive image processing based on input by user
• CPC Class Codes: G06T 2207/30024 Cell structures in vitro; Tissue sections in vitro
• CPC Class Codes: G06T 7/0012 Biomedical image inspection
• CPC Class Codes: G06V 20/693 Acquisition

Abstract

A display (34) provides from digital images of an excised tissue specimen the orientation and location of tissue and regions of interest (cancers). Images of slices through excised specimen are obtained using a confocal microscope (10) to obtain confocal maps to provide a mosaic of images each of which corresponds to a different map element. A macroscopic image of significant map elements and high resolution images of selected elements are provided to locate and mark regions which are cancerous thereby assisting the surgeon to excise such regions.

Claims

1. A system for optically sectioning and mapping a surgically excised tissue specimen comprising:

means for providing data representing a mosaic of optically formed sectional image elements of the specimen, wherein each of said sectional image elements has a field of view representing a plurality of pixels; and

means for viewing on a display at a first resolution a macroscopic composite image of the specimen in accordance with said data representing said mosaic of optically formed sectional image elements; and

means for selecting at least one location on the macroscopic composite image for enabling viewing by said viewing means of the sectional image elements associated with said location on said display at a second resolution higher than said first resolution, in which said macroscopic composite image exceeds the field of view of each of said sectional elements.

2. The system according to claim 1 further comprising means for providing anatomical maps of the specimen with areas containing cells which are cancerous marked to direct further surgical operations.

3. The system according to claim 1 wherein said data providing means comprises a laser scanning confocal microscope.

4. The system according to claim 1 wherein said data providing means comprises means for providing data respecting a plurality of said optically formed sectional images each corresponding to a different slice of said specimen, and said viewing means is operative to view a composite image and microscopic images of each of said slices.

5. The system according to claim 4 wherein said data providing means comprises means for scanning said specimen and providing said mosaic on each said slices during each of a plurality of scans.

6. The system according to claim 1 wherein said viewing means provided on said display said macroscopic composite image as a plurality of frames arranged according to said mosaic in which each of said frames represents at least one of said sectional image elements.

7. The system according to claim 3 wherein said confocal microscope has a stage, a cassette removably containing said specimen mounted on said stage and an objective lens, said lens and stage being movable with respect to each other along X-Y-Z coordinates during said mapping.

8. The system according to claim 1 wherein said data providing means comprises means providing data representing the orientation of said specimen with respect to a patient from which said specimen is excised.

28. The system according to claim 1 wherein said means further comprises means for enabling a user to identify the presence or absence of cancer in one or more of said sectional elements of the mosaic.

30. The system according to claim 1 wherein said viewing means further comprising viewing on said display said selected elements.

31. The system according to claim 1 wherein said means for providing data representing a mosaic of optically formed sectional image elements comprises means for automatically scanning an area of said excised tissue specimen to provide multiple sectional image elements on or within said excised tissue specimen, wherein said area exceeds the field of view of any one of said sectional image elements.

32. The system according to claim 31 wherein said automatically scanning of said area of said excised tissue specimen is at a user selected depth.

9. A method of optically sectioning and mapping tissue for histopathology comprising the steps of:

capturing an anatomic map of optically formed sectional image elements constituting a full field representation of a region of said tissue which constitutes a specimen, in which each of said sectional image elements has a field of view of said tissue;

storing data representing a plurality of pixels constituting each of said map elements and orientation of said specimen with respect to a patient;

displaying in response to said data a composite macroscopic image of said full field containing all of said pixels at a first resolution, in which said composite macroscopic image of said full field exceeds the field of view of each of said sectional image elements; and

displaying microscopic images of selected blocks of said elements of sufficient higher resolution than said first resolution to show portions of said specimen containing cells of interest.

10. The method according to claim 9 wherein said capturing step is carried out with the aid of a laser confocal microscope while scanning said specimen in a plurality of planes thereby providing a plurality of composite images and high resolution microscopic images for each of said composite images.

11. The method according to claim 9 further comprising the step of marking areas of said composite image corresponding to said blocks to indicate the histopathological characteristics of said blocks apparent from the microscopic images thereof.

22. The method according to claim 9 wherein said capturing step is carried out by one of confocal microscopy, optical coherence tomography, multi-photon microscopy, or high frequency ultrasound.

23. The method according to claim 9 wherein said capturing step is carried out by one of optical coherence tomography or multi-photon microscopy.

29. The method according to claim 9 further comprising the step of determining the presence or absence of cancer in one or more of said displayed microscopic images.

12. A system for optically sectioning and mapping tissue excised from a patient comprising:

means for capturing microscopic images of optically formed sections of the tissue;

means for presenting different parts of said tissue to said capturing means while maintaining the orientation of said tissue with respect to the patient from which said tissue was excised, operating said capturing means to capture at least one microscopic image of section of said tissue at each of said different parts of said tissue, and storing each of said captured microscopic images;

means for providing a macroscopic composite image of said specimen in accordance with a plurality of different ones of said stored microscopic images the specimen; and

means for displaying said composite image at a resolution to enable selection of one or more locations in said composite image and displaying at least one of said stored microscopic image associated with each said selected location at a higher resolution than the resolution of said displayed composite image, in which said macroscopic image exceeds the field of view of any one of said microscopic images.

13. The system according to claim 12 wherein said capturing means is operative by one of confocal microscopy, optical coherence tomography, multi-photon microscopy, or high frequency ultrasound.

14. The system according to claim 12 further comprising means for enabling a user to mark one or more of said selected locations of said composite image indicating histopathological characteristics.

15. The system according to claim 12 wherein said presenting means comprises:

a translation stage; and

means for mounting said tissue on said translation stage to present said tissue to said capturing means while maintaining the orientation of said tissue with respect to the patient from which said tissue was excised.

16. The system according to claim 15 wherein said mounting means is provided by a cassette containing said tissue located on said translation stage.

17. The system according to claim 12 wherein said composite image provides a map of the tissue and one or more of said microscopic images have sufficient resolution to show cells which are neoplastic.

18. The system according to claim 12 wherein said composite image provides a map to guide subsequent excisions in the patient.

19. The system according to claim 12 wherein said composite image is capable of being reoriented 180 degrees.

20. The system according to claim 12 wherein said means for displaying said composite image to enable selection of one or more locations in said composite image further comprises means for enabling a user to view live microscopic images captured by said capturing means at each said selected location, and to control imaging parameters of said capturing means to effect said live microscopic images captured.

21. The system according to claim 12 wherein said means for displaying said composite image to enable selection of one or more locations in said composite image further comprises means for enabling a user to view live microscopic images captured by said capturing means at each said selected location and to select the part of said tissue in said live microscopic images being presented to said capturing means.

24. A system for mapping tissue comprising:

an imager for capturing first data representing a mosaic of optically formed sectional microscopic images of the tissue; and

a computer system receiving said first data from said imager, and said computer system having memory storing at least second data representing a macroscopic image of said tissue in accordance with said first data, in which said macroscopic image has a field of view of said tissue greater in size than any one of said microscopic images, and said macroscopic image is displayable at a different resolution than said microscopic images.

25. The system according to claim 24 wherein said imager is operative by confocal microscopy.

26. The system according to claim 24 wherein said imager is operative by optical coherence tomography or multi-photon microscopy.

27. The system according to claim 24 wherein said imager is operative by high frequency ultrasound.