Sensor and detecting method

• US 7,195,738 B2
• Filed: 02/03/2005
• Issued: 03/27/2007
• Est. Priority Date: 02/03/2004
• Status: Expired due to Fees

• Current Assignee: Canon Kabushiki Kaisha (Canon Inc.)
• Original Assignee: Canon Kabushiki Kaisha (Canon Inc.)
• Inventors: Utsunomiya, Norihiko
• Primary Examiner(s): Le; Long V.
• Assistant Examiner(s): Jung; Unsu
• Application Number:
  US11/048,749
• Publication Number:
  US 20050170405A1
• Time in Patent Office: 782 Days
• Field of Search: 435/287.2, 435/288.3, 422/82.01
• US Class Current: 422/82.01
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0645 Electrodes
• CPC Class Codes: B01L 3/508 rigid containers not provided for above
• CPC Class Codes: G01N 27/3276 being a hybridisation with immobilised receptors using a FET type sensor G01N27/4145; concerning the hybridisation C12Q1/68
• CPC Class Codes: Y10S 436/806 Electrical property or magnetic property

Abstract

A sensor is provided for detecting a target substance in a sample by detecting a product formed in the presence of an enzyme and an enzyme substrate. The sensor comprises two or more working electrodes, a first capturing molecule immobilized on the working electrodes, and a current-detector for detecting a current value flowing through at least one of the working electrodes. The sensor also comprises a target substance detection means having an arithmetic unit for determining an amount of the target substance in the sample on the basis of the current value detected by the current-detector in a specified period of time, and a crosstalk detecting electrode arranged between the working electrodes for detecting a crosstalk between the working electrodes. The specified period of time is a time necessary for the current value through the crosstalk detecting electrode to show a particular change.

Claims

1. A sensor for detecting a target substance in a sample by detecting a product formed in the presence of an enzyme and an enzyme substrate comprising:

(A) two or more working electrodes;

(B) a first capturing molecule immobilized on the working electrodes to bind to the target substance;

(C) a current-detector for detecting a value of a current flowing through at least one of the working electrodes, when a composite comprising the first capturing molecule, the target substance, and a second capturing molecule modified with the enzyme has been formed on the working electrode, in order to detect the product formed when the enzyme substrate capable of acting with the enzyme is contacted with the enzyme;

(D) a target substance measuring apparatus having an arithmetic unit for determining an amount of the target substance in the sample based on the current value detected by the current-detector in a specified period of time after the enzyme has contacted with the enzyme substrate; and

(E) a crosstalk detecting electrode other than the working electrodes arranged between the working electrodes for detecting a crosstalk between the working electrodes, wherein the first capturing molecule is not immobilized on the crosstalk detecting electrode,

wherein the specified period of time is a time necessary for the current value through the crosstalk detecting electrode to exceed a particular value.

2. The sensor according to claim 1, wherein the target substance measuring apparatus determines a product amount from the current value, and determines the target substance amount in the sample from the product amount.

3. The sensor according to claim 1, wherein a different molecule is immobilized as a first capturing molecule on each of the working electrode.