Bovine germline D-genes and their application

• US 7,196,185 B2
• Filed: 05/06/2003
• Issued: 03/27/2007
• Est. Priority Date: 04/20/2001
• Status: Active Grant

• Current Assignee: Azad Kumar Kaushik, Farbod Shojaei, Surinder Singh Saini
• Original Assignee: Azad Kumar Kaushik, Farbod Shojaei, Surinder Singh Saini
• Inventors: Saini, Surinder Singh, Kaushik, Azad Kumar, Shojaei, Farbod
• Primary Examiner(s): Kaushal; Sumesh
• Assistant Examiner(s): Montanari; David A.
• Application Number:
  US10/429,979
• Publication Number:
  US 20030224433A1
• Time in Patent Office: 1,421 Days
• Field of Search: None
• US Class Current: 536/23.1
• CPC Class Codes: C07K 16/00 Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies antibodies with enzymatic activity, e.g. abzymes C12N9/0002
• CPC Class Codes: C07K 2317/565 Complementarity determining region [CDR]

Abstract

The present invention relates to a bovine VDJ cassette (BF1H1) that provides the novel ability to develop chimeric immunoglobulin molecule capable of incorporating both linear T cell epitope(s) (CDR1H and CDR2H) as well as conformational B cell epitope(s) (exceptionally long CDR3H). Further, multiple epitopes can be incorporated for development of multivalent vaccine by replacing at least a portion of an immunoglobulin molecule with the desired epitope such that functional ability of both epitope(s) and parent VDJ rearrangement is retained. The antigenized immunoglobulin incorporating both T and B epitopes of interest is especially useful for development of oral vaccines for use in humans apart from other species including cattle. The long CDR3H in BF1H1 VDJ rearrangement originates from long germline D-genes. The novel bovine germline D-genes provide unique molecular genetic marker for sustaining the D-gene pool in cattle essential for immunocompetence via selective breeding. D-gene specific DNA probe permits typing and selection of breeding cattle stock for maximum gemline D gene pool for better health and disease prevention. The bovine D-genes are unique to cattle and, therefore, provide sensitive and specific forensic analytical tool using molecular biology techniques to determine tissues suspected of bovine origin.

Claims

1. An isolated bovine germline D-gene comprising:

(a) the nucleic acid sequence as shown in SEQ ID NO;

2, SEQ ID NO;

3 or SEQ ID NO;

4 wherein T can also be U;

or

(b) the nucleic acid sequence that is fully complementary to the nucleic acid sequence of (a).

2. An isolated bovine germline D-gene according to claim 1 comprising the nucleic acid sequence of D-1 (SEQ ID NO:

2), D-2 (SEQ ID NO;

3) and/or D-3 (SEQ ID NO;

4).

5. An isolated plasmid comprising the nucleic acid sequence of claim 1.

3. An isolated bovine germline D-gene sequence comprising:

(a) the nucleic acid sequence as shown in SEQ ID NO;

6 wherein T can also be U;

or

(b) the nucleic acid sequence that is fully complementary to the nucleic acid sequence of (a).

6. An isolated plasmid comprising the nucleic acid sequence of claim 3.

4. An isolated bovine germline D sequence comprising the nucleic acid sequence of SEQ ID NO:

6.