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Immunoaffinity isolation of modified peptides from complex mixtures

  • US 7,198,896 B2
  • Filed: 06/19/2002
  • Issued: 04/03/2007
  • Est. Priority Date: 09/04/1998
  • Status: Expired due to Term
First Claim
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1. A method for isolating a target population of naturally-occurring post-translationally modified peptides from a complex mixture of peptides, said method comprising the steps of:

  • (a) obtaining a digested proteinaceous preparation from an organism, wherein said digested proteinaceous preparation comprises a complex in mixture of peptides comprising naturally-occurring post-translationally modified peptides from two or more different proteins;

    (b) contacting said digested proteinaceous preparation with at least one immobilized post-translational modification-specific antibody; and

    (c) isolating said target population of naturally-occurring post-translationally modified peptides specifically bound by said immobilized modification-specific antibody in step (b).

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