Multiplex detection compositions, methods, and kits
First Claim
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1. A method for determining the presence of at least one analyte in a sample, comprising:
- forming at least one molecular complex comprising (a) the at least one analyte and (b) at least one first probe comprising at least one reaction portion and at least one identity portion comprising at least one coded molecular tag; and
individually detecting the at least one molecular complex or at least part of the at least one molecular complex to determine the presence of the at least one analyte in the sample.
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Abstract
The present invention generally relates to the detection of analytes, particularly biomolecules in samples. The invention also relates to compositions, methods, and kits for detecting the presence of analytes, typically in multiplex detection formats. The invention also relates to methods for determining the presence of at least one analyte in a sample, the methods employing employ single molecule detection techniques to individually detect at least one molecular complex or at least part of a molecular complex.
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Citations
28 Claims
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1. A method for determining the presence of at least one analyte in a sample, comprising:
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forming at least one molecular complex comprising (a) the at least one analyte and (b) at least one first probe comprising at least one reaction portion and at least one identity portion comprising at least one coded molecular tag; and individually detecting the at least one molecular complex or at least part of the at least one molecular complex to determine the presence of the at least one analyte in the sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28)
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Specification