Apparatus for interrogating an addressable array
First Claim
1. An apparatus for interrogating an addressable array of multiple features of different biopolymenc moieties, comprising:
- (a) an adjustable detection angle detector system comprising more than one detector each of which is aligned with an emission filter that filters out light of an interrogating wavelength such that each detector of the detection system detects light of different wavelengths at respective different detection angles with an optical axis aligned at each detection angle, wherein said system is configured for positioning at least one of said more than one detectors at a site for receiving a constructively interfering emission from an array having a reflective coating, wherein said system is further configured for determining said position for receiving a constructively interfering emission; and
(b) a processor programmed to receive signals from the detector system and correlate the received signals with respective biopolymeric features of the array.
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Abstract
A method and apparatus of interrogating an addressable array unit, which includes a substrate, a light reflecting layer on a front side of the substrate, and a plurality of features on a front side of the array. The method may include, for each of multiple features, illuminating the feature simultaneously with reflected and non-reflected interrogating light. A light emitted from respective features is detected. Either or both, constructive interference of interrogating light at the features, or constructive interference of light emitted from the features, can be obtained to allow lowering of light power from the source, enhanced signal, or reduced noise, or combinations of the foregoing. High depth discrimination may also be obtained without the need for a confocal detection system with conventional pinhole.
116 Citations
7 Claims
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1. An apparatus for interrogating an addressable array of multiple features of different biopolymenc moieties, comprising:
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(a) an adjustable detection angle detector system comprising more than one detector each of which is aligned with an emission filter that filters out light of an interrogating wavelength such that each detector of the detection system detects light of different wavelengths at respective different detection angles with an optical axis aligned at each detection angle, wherein said system is configured for positioning at least one of said more than one detectors at a site for receiving a constructively interfering emission from an array having a reflective coating, wherein said system is further configured for determining said position for receiving a constructively interfering emission; and (b) a processor programmed to receive signals from the detector system and correlate the received signals with respective biopolymeric features of the array. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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Specification