Process for the production of proteins and the production of arrays of proteins
First Claim
1. A process for the production of at least one protein of interest comprising culturing a host cell in a compartment system, which host cell is stably expressing a secretion system and capable of secreting the heterologous protein of interest, wherein the protein of interest is secreted by the host cell by means of a secretion signal, and which compartment system has at least a first and a second compartment, and wherein the host cell is located in the first compartment, and wherein the first and second compartment are separated from each other by a barrier, wherein the barrier is permeable for the secreted protein of interest, but not permeable for the host cell.
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Abstract
Described is a novel process for the production of at least one protein of interest by secretion of the protein of interest from a pro- or eukaryotic host cell in a compartment system, which host cell is stably expressing a secretion system and capable of heterologous secretion of the protein of interest and which compartment system has at least a first and a second compartment and wherein the host cell is located in the first compartment and wherein the first and second compartment are separated from each other by a barrier, wherein the barrier is permeable for the secreted protein of interest, but not permeable for the host cell.
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Citations
16 Claims
- 1. A process for the production of at least one protein of interest comprising culturing a host cell in a compartment system, which host cell is stably expressing a secretion system and capable of secreting the heterologous protein of interest, wherein the protein of interest is secreted by the host cell by means of a secretion signal, and which compartment system has at least a first and a second compartment, and wherein the host cell is located in the first compartment, and wherein the first and second compartment are separated from each other by a barrier, wherein the barrier is permeable for the secreted protein of interest, but not permeable for the host cell.
- 14. A process for the production of an array of several different proteins of interest, comprising culturing host cells in a compartment system, which host cells are stably expressing a secretion system, wherein the proteins of interest are secreted by the host cell by means of secretion signals, which enables the host cells to secrete the respective heterologous protein of interest and which compartment system has at least a first and a second compartment and wherein the different host cells are arranged in the form of an array in said first compartment and wherein the first and second compartment are separated from each other by a barrier, wherein the barrier is permeable for the secreted proteins of interest, but not permeable for the host cell and wherein the secreted proteins of interest are received in the second compartment in the form of an array.
Specification