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Method of cryopreserving selected sperm cells

DC CAFC
  • US 7,208,265 B1
  • Filed: 01/05/2000
  • Issued: 04/24/2007
  • Est. Priority Date: 11/24/1999
  • Status: Expired due to Term
First Claim
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1. A method of cryopreserving sex-selected sperm cells, comprising:

  • a. obtaining sperm cells from a species of a non-human male mammal;

    b. sorting said sperm cells, without the presence of protective compounds in seminal plasma, and based upon sex-type to provide a collection of sex-selected sperm cells obtained using flow cytometry or fluorescence-activated cell sorting;

    c. cooling said sex-selected sperm cells;

    d. suspending said sex-selected sperm cells in an extender to provide a concentration of sperm cells of about 5 million per milliliter of extender to about 10 million per milliliter of extender; and

    e. freezing said sex-selected sperm cells in said extender.

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