Methods of increasing lean tissue mass using OB protein compositions

• US 7,208,577 B2
• Filed: 01/11/2005
• Issued: 04/24/2007
• Est. Priority Date: 11/22/1995
• Status: Expired due to Fees

• Current Assignee: Amgen Inc.
• Original Assignee: Amgen Inc.
• Inventors: Pelleymounter, Mary Ann, Toombs, Christopher Francis, Mann, Michael Benjamin
• Primary Examiner(s): Chan; Christina
• Assistant Examiner(s): HUYNH, PHUONG N
• Application Number:
  US11/033,600
• Publication Number:
  US 20050176107A1
• Time in Patent Office: 833 Days
• Field of Search: 530/350, 530/387.3, 424/192.1, 424/194
• US Class Current: 530/350
• CPC Class Codes: A61K 38/2264 Obesity-gene products, e.g. leptin
• CPC Class Codes: C07K 14/5759 Products of obesity genes, e.g. leptin, obese (OB), tub, fat
• CPC Class Codes: C07K 2319/30 Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto

Abstract

The present invention provides methods of creating and using OB protein compositions with an antibody constant region or portion thereof fused to an OB protein. The fusion protein is created by attaching the polyamino acids to the OB protein moiety. The fusion proteins can then be used for various therapeutic uses.

Claims

1. An isolated fusion protein comprising an Fc portion of an antibody attached at the N-terminus of an OB protein of SEQ ID NO:

2.

2. An isolated fusion protein comprising an Fc portion of an antibody attached at the N-terminus of an OB protein of SEQ ID NO:

4.

3. The isolated fusion protein of claim 2, wherein, in the 146 residue sequence of SEQ ID NO:

4, lysine is substituted at position 35 and isoleucine is substituted at position 74.

4. The isolated fusion protein of claim 2, wherein the Fc portion and the OB protein are attached via a linker.