Composite materials for controlled release of water soluble products

• US 7,211,275 B2
• Filed: 01/13/2005
• Issued: 05/01/2007
• Est. Priority Date: 01/16/2004
• Status: Expired due to Fees

• Current Assignee: Massachusetts Institute of Technology
• Original Assignee: Massachusetts Institute of Technology
• Inventors: Ying, Jackie Y., Yong, Tseh-Hwan
• Primary Examiner(s): Wax; Robert A.
• Application Number:
  US11/034,217
• Publication Number:
  US 20060024377A1
• Time in Patent Office: 838 Days
• Field of Search: None
• US Class Current: 424/462
• CPC Class Codes: A61K 9/143 with inorganic compounds
• CPC Class Codes: A61K 9/146 with organic macromolecular compounds
• CPC Class Codes: A61L 2300/252 Polypeptides, proteins, e.g. glycoproteins, lipoproteins, cytokines
• CPC Class Codes: A61L 2300/258 Genetic materials, DNA, RNA, genes, vectors, e.g. plasmids
• CPC Class Codes: A61L 2300/404 Biocides, antimicrobial agents, antiseptic agents
• CPC Class Codes: A61L 2300/412 Tissue-regenerating or healing or proliferative agents
• CPC Class Codes: A61L 2300/416 Anti-neoplastic or anti-proliferative or anti-restenosis or anti-angiogenic agents, e.g. paclitaxel, sirolimus
• CPC Class Codes: A61L 2300/426 Immunomodulating agents, i.e. cytokines, interleukins, interferons
• CPC Class Codes: A61L 2300/602 Type of release, e.g. controlled, sustained, slow
• CPC Class Codes: A61L 27/46 with phosphorus-containing inorganic fillers
• CPC Class Codes: A61L 27/54 Biologically active materials, e.g. therapeutic substances A61L27/227 takes precedence
• CPC Class Codes: A61L 27/58 Materials at least partially resorbable by the body
• CPC Class Codes: C12N 2501/155 Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
• CPC Class Codes: C12N 2533/30 Synthetic polymers thermoreactive polymers, e.g. PNIPAm, C12N2539/10
• CPC Class Codes: C12N 2533/40 Polyhydroxyacids, e.g. polymers of glycolic or lactic acid (PGA, PLA, PLGA); Bioresorbable polymers
• CPC Class Codes: C12N 5/0655 Chondrocytes; Cartilage

Abstract

Composite materials comprising a water-soluble compound adsorbed onto a basic inorganic material and a bio-degradable polymer which yields acidic degradation products, methods of producing same, and methods of use thereof are described, wherein the composite materials are designed so as to provide controlled release of the water soluble molecule.

Claims

1. A controlled release composite comprising:

a basic inorganic material;

a water-soluble compound adsorbed to said basic inorganic material; and

a bio-degradable polymer which yields acidic degradation products

whereby acidic degradation products from said polymer facilitate dissolution of said basic inorganic material and release of said adsorbed compound.

2. The controlled release composite of claim 1, wherein said water-soluble compound is a peptide, a protein, a nucleic acid or a drug.

3. The controlled release composite of claim 1, wherein said basic inorganic material is hydroxyapatite, carbonated apatite, calcium phosphate, calcium carbonate, zinc oxide, magnesium oxide, or alumina.

4. The controlled release composite of claim 1, wherein said bio-degradable polymer is a poly(α

-hydroxyacid), such as poly(lactic acid), poly(glycolic acid), or blends thereof, a poly(orthoester), poly(anhydride), or a poly(hydroxyl alkanoate).

5. The controlled release composite of claim 1, wherein dissolution of said basic inorganic material releases charged species over a course of time.

6. The controlled release composite of claim 5, wherein said charged species comprises calcium or phosphate, or a combination thereof.

7. The controlled release composite of claim 1, wherein said system may be in the form of a particle.

8. The controlled release composite of claim 1, wherein said composite is in the form of a gel, bulk scaffold, thin film or pellet.

9. The controlled release composite of claim 1, further comprising an amphoteric species, which alters the rate of dissolution of said basic inorganic material, liberation of said adsorbed compound, or a combination thereof.

10. The controlled release composite of claim 9, wherein said amphoteric species serves as a diffusion barrier to the liberated compound.

11. The controlled release composite of claim 10, wherein said amphoteric species is a gelatin.

12. The controlled release composite of claim 1, wherein said biodegradable polymer comprises a blend of at least 2 polymers, which differ in their hydrophobicity.

13. The controlled release composite of claim 12, wherein varying the concentration of said at least 2 polymers affects the release rate of said water-soluble compound.

14. The controlled release composite of claim 1, further comprising unadsorbed basic inorganic material.

15. A process for preparing a composite scaffolding comprising a water-soluble compound, comprising the steps of:

(a) adsorbing a water-soluble compound onto a basic, inorganic material;

(b) combining the product in (a) with a solution of a biodegradable polymer which yields acidic degradation products to form a solid-in oil emulsion;

(c) precipitating the composite scaffolding product in (b); and

(d) isolating said composite scaffolding.

16. The process of claim 15, wherein said water-soluble compound is a peptide, a protein, a nucleic acid or a drug.

17. The process of claim 15, wherein said basic inorganic material is hydroxyapatite or carbonated apatite.

18. The process of claim 15, wherein said bio-degradable polymer is polylactide-co-glycolide.

19. The process of claim 15, wherein isolation of said composite scaffolding comprises drying or lyophilization.

20. The process of claim 15, wherein said polymer solution concentration, molecular weight of said polymer, or a combination thereof, may be varied.

21. The process of claim 20, wherein varying said polymer solution concentration, molecular weight of said polymer, or a combination thereof, produces composite microparticles differing in size.

22. The process of claim 15, wherein said polymer comprises a blend of at least 2 polymers, which differ in their hydrophobicity.

23. The process of claim 22, wherein said blend of at least 2 polymers may be varied in terms of their concentration.

24. The process of claim 23, wherein varying the concentration of said at least 2 polymers affects polymer degradation rate in said scaffolding.

25. The process of claim 15, wherein free apatite is added in step (b).

26. The process of claim 15, wherein the percent water-soluble compound adsorbed onto said basic, inorganic material is varied.

27. The process of claim 26, wherein varying said percent water-soluble compound adsorbed onto said basic, inorganic material affects the release kinetics of said water-soluble compound from said composite scaffolding.

28. The process of claim 15, further comprising the step of combining said composite microparticles with a solution of gelatin, chitosan, arabinogalactan, collagen, alginate, hyaluronic acid, fibrin, or cellulose.

29. The process of claim 28, further comprising the step of adding a cross-linker.

30. The process of claim 29, wherein said cross-linker is glutaraldehyde.

31. A porous scaffolding prepared by the process of claim 15.

32. A method of controlled delivery of a therapeutic water-soluble compound to a subject, comprising administering to said subject a controlled release composite comprising a therapeutic water-soluble compound adsorbed onto a basic, inorganic material and a bio-degradable polymer which yields acidic degradation products, whereby degradation of said polymer yields acidic degradation products therefrom, which stimulates dissolution of the inorganic material and release of said therapeutic water-soluble compound over a period of time, thereby being a method of controlled delivery of a therapeutic water-soluble compound.

33. The method of claim 32, wherein said therapeutic compound is a peptide, a protein, a nucleic acid or a drug.

34. The method of claim 32, wherein said therapeutic compound is osteogenic, chondrogenic or angiogenic.

35. The method of claim 32, wherein said therapeutic compound is an antibacterial, antiviral, antifungal or antiparasitic compound.

36. The method of claim 32, wherein said therapeutic compound is immunostimulatory.

37. The method of claim 32, wherein said therapeutic compound inhibits inflammatory or immune responses.

38. The method of claim 32, wherein said basic inorganic material is hydroxyapatite, carbonated apatite, calcium phosphate, calcium carbonate, zinc oxide, magnesium oxide, or alumina.

39. The method of claim 32, wherein said bio-degradable polymer is a poly(α

-hydroxyacid), such as poly(lactic acid), poly(glycolic acid), or blends thereof, a poly(orthoester), poly(anhydride), or a poly(hydroxy alkanoate).

40. The method of claim 32, wherein dissolution of said basic inorganic material releases charged species over a course of time.

41. The method of claim 32, wherein said charged species comprises calcium or phosphate, or a combination thereof.

42. The method of claim 32, wherein said composite is in the form of a gel, bulk scaffold, thin film or pellet.

43. The method of claim 32, wherein said composite controlled release system further comprises an amphoteric species, which alters the rate of dissolution of said basic inorganic material, liberation of said adsorbed compound, or a combination thereof.

44. The method of claim 42, wherein said amphoteric species serves as a diffusion barrier to the liberated compound.

45. The method of claim 42, wherein said amphoteric species is a gelatin.

46. The method of claim 32, wherein said controlled release composite comprises a biodegradable polymer whose concentration, molecular weight, or combination thereof, is varied.

47. The method of claim 45, wherein varying said biodegradable polymer concentration, molecular weight, or combination thereof, affects the release rate of said water-soluble compound.

48. The method of claim 32, wherein said biodegradable polymer comprises a blend of at least 2 polymers, which differ in their hydrophobicity.

49. A method of tissue engineering comprising the step of contacting a cell with a composite scaffolding comprising a water-soluble tissue-promoting factor adsorbed onto a basic, inorganic material and a bio-degradable polymer which yields acidic degradation products, whereby degradation of said polymer yields acidic degradation products therefrom, which stimulates dissolution of said inorganic material and release of said water-soluble tissue-promoting factor, and whereby said cell responds to said tissue-promoting factor, promoting tissue formation, thereby providing a method of tissue engineering.

50. The method of claim 49, wherein said tissue is bone or cartilage.

51. The method of claim 49, wherein said tissue-promoting factor is a bone morphogenetic protein, a member of the TGF-β

superfamily, or a member of the BMP signaling cascade.

52. A method of stimulating or enhancing bone repair, or maintaining or increasing bone volume, bone quality, or bone strength in the body of a subject in need, comprising the steps of:

contacting a cell in said subject with a composite controlled release system comprising a water-soluble factor adsorbed onto a basic, inorganic material and a bio-degradable polymer which yields acidic degradation products, whereby degradation of said polymer yields acidic degradation products therefrom, which stimulates dissolution of said inorganic material and release of said water-soluble factor, and whereby said cell responds to said factor, promoting bone repair, increased bone volume, bone quality, or bone strength, thereby providing a method of stimulating or enhancing bone repair, or maintaining or increasing bone volume, bone quality, or bone strength in the body of a subject in need.

53. A method of nucleic acid delivery, comprising contacting a cell with a composite controlled release system comprising a nucleic acid adsorbed onto a basic, inorganic material and a bio-degradable polymer which yields acidic degradation products, whereby degradation of said polymer yields acidic degradation products therefrom, which stimulates dissolution of the inorganic material and release of said nucleic acid, thereby being a method of nucleic acid delivery.