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Primitive neural stem cells and method for differentiation of stem cells to neural cells

  • US 7,211,434 B2
  • Filed: 09/28/2001
  • Issued: 05/01/2007
  • Est. Priority Date: 09/29/2000
  • Status: Expired due to Fees
First Claim
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1. A method for differentiating one or more pluripotent mammalian embryonic stem (ES) cells comprising:

  • a. culturing the mammalian ES cells at low cell density in a serum-free and feeder-layer free media comprising leukemia inhibitory factor; and

    b. allowing said mammalian ES cells to differentiate to primitive neural stem cells.

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