Device and method for multiple analyte detection
First Claim
1. A method for detecting or quantitating one or more polynucleotide sequences in a sample, said method comprisingproviding a device comprising a substrate defining a sample-distribution network including (i) a sample inlet, (ii) two or more detection chambers, and (iii) channel means providing a dead-end fluid connection between each of said chambers and said inlet, wherein at least two of said detection chambers each contain pre-loaded therein, at least one different, sequence-specific polynucleotide binding polymer for detecting or quantitating different polynucleotide sequences that may be present in such sample, to produce a detectable signal,applying the sample to the sample inlet and delivering the sample into the network by action of a force whereby the sample is delivered to the detection chambers,allowing the delivered sample to react with the at least one different, sequence-specific polynucleotide binding polymer in each detection chamber under conditions effective to produce a detectable signal in each detection chamber when the one or more polynucleotide sequences are present in the sample, andmeasuring the detectable signal to detect or quantitate the one or more polynucleotide sequences in the sample.
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Abstract
The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid sample. The device includes a substrate which defines a sample-distribution network having (i) a sample inlet, (ii) one or more detection chambers, and (iii) channel means providing a dead-end fluid connection between each of the chambers and the inlet. Each chamber may include an analyte-specific reagent effective to react with a selected analyte that may be present in the sample, and detection means for detecting the signal. Also disclosed are methods utilizing the device.
79 Citations
20 Claims
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1. A method for detecting or quantitating one or more polynucleotide sequences in a sample, said method comprising
providing a device comprising a substrate defining a sample-distribution network including (i) a sample inlet, (ii) two or more detection chambers, and (iii) channel means providing a dead-end fluid connection between each of said chambers and said inlet, wherein at least two of said detection chambers each contain pre-loaded therein, at least one different, sequence-specific polynucleotide binding polymer for detecting or quantitating different polynucleotide sequences that may be present in such sample, to produce a detectable signal, applying the sample to the sample inlet and delivering the sample into the network by action of a force whereby the sample is delivered to the detection chambers, allowing the delivered sample to react with the at least one different, sequence-specific polynucleotide binding polymer in each detection chamber under conditions effective to produce a detectable signal in each detection chamber when the one or more polynucleotide sequences are present in the sample, and measuring the detectable signal to detect or quantitate the one or more polynucleotide sequences in the sample.
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20. A method for analyzing one or more polynucleotide sequences in a sample, said method comprising
providing a device comprising a substrate defining a (i) a sample inlet, (ii) a plurality of detection chambers, and (iii) channel structure providing a dead end fluid connection between each of said chambers and said inlet, wherein two or more of said detection chambers contain pre-loaded therein, one or more sequence-specific polynucleotide binding polymers for the analysis of one or more polynucleotide sequences that may be present in such sample, to produce a detectable signal, applying the sample to the sample inlet and delivering the sample to the detection chambers, allowing the delivered sample to react with at least one of the one or more sequence-specific polynucleotide binding polymers in at least one of said detection chambers under conditions effective to produce a detectable signal when the one or more polynucleotide sequences are present in the sample, and detecting for said signal.
Specification